Genomic Technology

Question 1

The process in which a DNA fragment is inserted into a DNA molecule via a vector and can replicate independently in a host cell

Answer 1

molecular cloning

Question 2

Vector designed for up to 50 kbs

Answer 2

Cosmids

Question 3

Vector designed for 150 to 300 kbs

Answer 3

Bacterial Artificial Chromosomes (BACs)

Question 4

Vector designed for 200 to 500 kbs

Answer 4

Yeast Artificial Chromosomes (YACs)

Question 5

Can RNA be cloned directly?

Answer 5

Nah son

Question 6

In order to be cloned, RNA must first be copied by…

Answer 6

Reverse transcriptase, resulting in cDNA.

Question 7

What is the different between regular DNA and cDNA

Answer 7

cDNA does not include introns

Question 8

How would you sequence the epigenome?

Answer 8

bisulfite sequencing

Question 9

How does bisulfite sequencing work?

Answer 9

It converts C’s to U’s, then you look for the changes. The 5-methylcytosines would not have changed.

Question 10

What does metagenomics, examine in order to understand the microbiome?

Answer 10

conserved 16S ribosomal RNA, used as a diagnostic tool to identify bacteria species

Question 11

Southern blotting

Answer 11

Cloned DNA can be labeled with radioactive nucleotides (or modified to fluoresce). Labeled DNA is used as a probe to hybridize with complementary DNA or RNA.

Question 12

What are chimeric offspring?

Answer 12

mixture of cells arising from normal and transfected embryonic cells

Question 13

A method of gene knockout taking advantage of site-specific recombinases to introduce gene deletions, inversions or translocations.

Answer 13

Cre-Lox System

Question 14

Most popular restriction endonuclease which recognizes GAATTC

Answer 14

EcoRI

Question 15

What looks at DNA-protein interactions?

Answer 15

Chip Seq