Genetic Modification Of Plasmids Flashcards
1
Q
What is genetic engineering
A
The process of manually transferring genetic information from one organism to another
2
Q
Where is DNA found in bacteria
A
A ring of DNA called a plasmid
3
Q
What are plasmids created in a lab called
A
Constructs or vectors
4
Q
List the parts of a plasmid
A
- Origin of replication
- Antibiotic resistant genes
- Selectable markers
- Promoter
- Restriction site
- Inserted gene
5
Q
What is the role of a promoter
A
Allows for gene transcription
6
Q
What is the origin of replication
A
A sequence of DNA at which replication is initiated on a chromosome, plasmid or virus
7
Q
What is an antibiotic resistant gene
A
It allows bacteria to survive the effects of antibiotics
8
Q
What is a multiple cloning site
A
A region in a plasmid or vector where multiple restriction enzyme recognition sites are located
9
Q
What is an insert
A
The genetic element that you wish to study using a particular plasmid
