Gene Technology Flashcards
1
Q
what is the name of the enzyme that is used to cut sections of DNA?
A
-restriction endonuclease
2
Q
how does restriction endonuclease cut sections of DNA?
A
-through a hydrolysis reaction at specific sequences of bases called recognition sequences
3
Q
what are the two cuts that restriction enzymes can make?
A
-blunt cut where it is cut straight across two bases
-sticky ends where the cut is staggered
4
Q
what are sticky ends?
A
-short sections of DNA where a small number of bases are exposed and unpaired
5
Q
A
