Gene Expression Flashcards

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1
Q

Which enzyme is responsible for transcription?

A

RNA polymerase

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2
Q

Which process produces a primary mRNA transcript?

A

Transcription

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3
Q

Which process takes place to turn a primary mRNA transcript into a mature mRNA molecule?

A

Splicing

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4
Q

Which process is when the mature mRNA is used to direct the synthesis of a protein?

A

Translation

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5
Q

Which process takes place after translation and can result in multiple different proteins being produced from one gene?

A

Post-translational modification

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6
Q

Where does transcription take place?

A

In the nucleus

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7
Q

Where does translation take place?

A

At the ribosome

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8
Q

Which molecule carries specific amino acids to the ribosome?

A

tRNA

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9
Q

What is the ribosome composed of?

A

rRNA and protein

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10
Q

What is the function of RNA polymerase?

A

Breaks the hydrogen bonds between strands of DNA to begin transcription and adds complementary nucleotides to build a molecule of mRNA.

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11
Q

Describe the complementary base pairing used to build a molecule of RNA.

A

A—U, C—G

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12
Q

Which bonds hold amino acids together to form a polypeptide?

A

Peptide bond

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13
Q

What happens during splicing?

A

Introns are removed and exons are spliced together.

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14
Q

Which codons begin and end translation?

A

START and STOP codons.

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15
Q

What is the function of tRNA?

A

Carries a specific amino acid and has an anticodon which is complementary to a codon.

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16
Q

What is the function of mRNA?

A

It carries a complementary copy of the DNA code from the nucleus to a ribosome in the cytoplasm.

17
Q

What happens during post-translational modification?

A

The addition of phosphate or carbohydrate molecules, cleavage of polypeptide chains.

18
Q

What is alternative splicing?

A

When introns are treated as exons or exons are treated as introns leading to different versions of the mature mRNA

19
Q

How can one gene lead to the production of many proteins?

A

Post-translational modification, alternative splicing

20
Q

Which bonds are responsible for the final shape of a protein?

A

Hydrogen bonds between amino acids and sulphur bridges.

21
Q

What is an intron?

A

A non-coding region of mRNA

22
Q

What is an exon?

A

Coding region of mRNA.

23
Q

Which factors can regulate gene expression?

A

Intracellular and extracellular factors.

24
Q

How is gene expression controlled?

A

By regulating transcription or translation.

25
Q

What is the phenotype of an organism?

A

: The physical and chemical characteristics of an organism.

26
Q

What happens during translation at the ribosome?

A

tRNA with complementary anticodons match with codons on the mRNA

27
Q

What are the differences between RNA and DNA nucleotides?

A

RNA contains RIBOSE SUGAR and URACIL, DNA contains DEOXYRIBOSE SUGAR and THYMINE

Also, RNA is one strand whereas DNA has two strands

28
Q

What is a codon?

A

A sequence of three bases on mRNA which codes for one amino acid.