Fundamentals of microscopy

Question 1

what are five types of light microscope

Answer 1

• Bright field microscope (Classical light microscope)
• Fluorescence microscope
• Phase-Contrast microscope
• Confocal microscope
• Polarizer (Polarizer) Light microscope

Question 2

what are the two parts of a light microscope

Answer 2

support part 
optical part (functional part)

Question 3

what is the support part composed of

Answer 3

base, arm, stage, tube, coarse focus and fine focus

Question 4

what is the optical part composed of

Answer 4

diaphragm, condenser, objective lens and ocular lens

Question 5

what is the function of the diaphragm

Answer 5

collects the incident light

Question 6

what is the main task of the condenser

Answer 6

collect the light on the slide and illuminate it sufficiently

Question 7

where is the image first formed

Answer 7

ocular lens

Question 8

ability to distinguish between two objects is…..

Answer 8

resolution

Question 9

what is the ocular magnification

Answer 9

x10

Question 10

what are tissue sections usually excited by when viewed under a Fluorescence Microscope

Answer 10

excited by ultraviolet (ultraviolet-UV) light

Question 11

what must Fluorescence Microscopes have

Answer 11

UV light source and special filters that select the light of different wavelengths emitted by substances

Question 12

what microscope doesn’t require detection or staining

Answer 12

Phase-contrast microscopes

Question 13

what principle is the phase contrast based on

Answer 13

that light changes speed as it passes through cellular and extracellular structures, depending on different refractive indices

Question 14

what are the two types of electron microscopy

Answer 14

• Transmission Electron Microscope (TEM)

* Scanning Electron Microscopy (SEM)

Question 15

what transmits the emitted electrons to the anode with an accelerating voltage of 60-120 kV on a Transmission Electron Microscope (TEM)

Answer 15

Tungsten wire (electron gun, cathode) at the top of a vacuum column

Question 16

Electron-permeable fields appear……… and electrons are deflected appear……… on a Transmission Electron Microscope (TEM)

Answer 16

brighter

darker

Question 17

in the Scanning Electron Microscope how many dimensions are external surfaces examined in

Answer 17

in 3 dimensions

Question 18

in the Scanning Electron Microscope what is first done to the sample

Answer 18

first dried and covered with a thin layer, especially with gold palladium, so that it does not pass electron

Question 19

true or false, The thin electron beam focuses on the sample and passes through the sample

Answer 19

false, it only focuses on the sample, the TEM passes through the sample though

Question 20

what are electrons captured by

Answer 20

a sensor