fundamental review 2 Flashcards

1
Q

what is transcription?

A

the process by which dna is making RNA

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2
Q

what is translation?

A

the process by which proteins are coded for RNA

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3
Q

what protien is responsible for dna synthesis?

A

dna polymerase

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4
Q

how accurate is dna synthesis?

A

it is very accurate, only with a few errors per cell

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5
Q

how does the dna polymerase monitor for abnormalities?

A

there is a proofreading polypeptide present in the polymerase

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6
Q

what does this proofreading polymerase do for the gene sequence?

A

it can allow the wrong base to be excluded

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7
Q

what comes first, transcription or translation?

A

transCription
(remember that c comes before l in the alphabet)

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8
Q

does DNA need a primer to begin replication

A

yes it does need a primer

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9
Q

what enzyme does transcription?

A

the RNA polymerase

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10
Q

how many strands of DNA acts as the template for transcription?

A

just one strand

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11
Q

after translation, does the new sequence remail attatched to the strand of DNA?

A

no, it does not. after a few pairs, the strand of dna is deplaced by the polymerase

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12
Q

is transcription or or less accurate than dna replication?

A

less accurate. there is more space for abnormalities

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13
Q

what is the general process of transcription?

A

the rna polymerase binds to the dna, it elongates, then it terminates

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14
Q

what is translation?

A

when mature RNA is made into protein

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15
Q

does translation take minimal or a lot of energy?

A

it takes a lot of energy and is very taxing on the cell

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16
Q

what is the purpose of tRNA?

A

to be an adaptor for the growing rna chain and the amino acid

17
Q

is the entire portion of Mrna translated?

A

no, just a section of the strand

18
Q

what is a codon?

A

a 3 letter section of rna that tells which amino acid is going to be coded

19
Q

what is the open reading frame?

A

the section of rna that is the initiating codon (start codon), and it is usually AUG

20
Q

what is the kozak sequence?

A

the section of the gene that determines where the start AUG is

21
Q

what is the general rule for trna base pairs and the ending rule ?

A

5’, (varying sequences), CCA, 3’

22
Q

what is always going to be the ending of the trna sequence?

23
Q

what is the purpose of the ribosome?

A

to direct protien from the synthesis of mrna

24
Q

how many proteins can a ribosome direct at any given time?

A

one at a time

25
can different proteins be directed by different ribosomes at any given time?
yes
26
how does termination occur?
when the ribosome gets to the stop codon
27
can ribosomes be recycled?
yes they are and they can be used for other protien sequences
28
are codons/amino acids overlapping?
in most cases no
29
what is the chain of codons called?
a frame
30
what is a missense mutation?
when a single base pair mutation causes one amino acid to be swapped for another
31
what is a nonsense mutation?
when the mutation causes an amino acid to be changed to a stop codon
32
what is a frameshift mutation?
when a single base pair is inserted or deleted and this causes the entire frame is read as a different one