Functional Characterisation of CYP

Question 1

The leveling off of the reaction at high substrate concentrations, reflecting the saturation of binding site of enzymes with substrate

Indicator of catalytic efficiency

Answer 1

Vmax

Question 2

Reflect the relative affinity of the enzyme for the sybstrate

Answer 2

Km

Question 3

Emzyme-mediated clearance that would occur without physiological limitations

Answer 3

CL int

Question 4

CYP is a superfamily of _____, which consists of _______ and a tightly bound prosthetic ______.

Answer 4

hemoproteins, apoprotein, haem group

Question 5

Substrate of CYP2C19

Answer 5

S- mephenytoin

Omeprazole

Citalopram

Diazepram

Proguanil

Question 6

CYP2C19 polymorphism:

Extensive metaboliser

Answer 6

CYP2C19*1

wild type

Question 7

CYP2C19 polymorphism:

Poor metaboliser

Answer 7

CYP2C192 and CYP2C193

Question 8

CYP2C19 polymorphism:

Ultrarapid metaboliser

Answer 8

CYP2C19*17

Question 9

deleterious mutations of CYP2C19

Answer 9

CYP2C1923, CYP2C1924, CYP2C19*25

Question 10

Drug failure of failure of activation of prodrug and which catagory it belongs?

Answer 10

Clopidogrel, PM