from review worksheet

Question 1

RISC

Answer 1

RNA induced silencing complex

plays central roll in post-transcription gene regulation through mRNA cleavage, degradation, and/or translational repression

Question 2

what process is Lac2 used in

Answer 2

molecular cloning

Question 3

ways cyclin-Cdk can be regulated

Answer 3

CKI (cyclin cdk inhibitor proteins)

activator + inhibitory kinases

Question 4

advantages of operon transcription (bacteria) versus eukaryotic version of group gene regulation

Answer 4

operon: requires less regulatory sequences and in turn a shorter genome

eukaryotes: more options, genes can be regulated in groups or individually

Question 5

transgene

Answer 5

gene artifically intrduced into genome of another organism

Question 6

what is P53 and what does it do when phosphorilated

Answer 6

is a transcription factor when phosphorilated.

when active, it upregulates transcription of genes that code for cell cycle + cell death (including c-cdk inhibitors)

genes controlled by p53 would then be flourezcent when activated and transcribed