From DNA to Protein Flashcards

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1
Q

What 2 processes take DNA to Protein

A
  1. Transcription (RNA synthesis)

2. Translation (Protein synthesis)

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2
Q

Transcription

A

DNA—RNA

  1. Initiation
  2. Elongation
  3. Termination
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3
Q

what are the 3 parts to each gene?

A
  • promoter region
  • body
  • termination signal
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4
Q

promoter region of a gene

A

where RNA polymerase binds

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5
Q

body of a gene

A

begins with the start codon

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6
Q

termination signal of a gene

A

end codon and signal for RNA polymerase to detach

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7
Q

What is the Machinery for transcription

A
  • transcription factors

- RNA Polymerase

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8
Q

Transcription factors

A

recognize promoter region and signals the polymerase to bind

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9
Q

RNA Polymerase

A

pulls DNA strands apart; creates RNA strand from the DNA template strand

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10
Q

Initiation of transcription

A
  • transcription factors find and attach to the promoter
  • RNA polymerase attaches to DNA
  • Polymerase separates DNA and begins synthesis of RNA from DNA template strand
  • -reads 3 to 5 prime
  • -creates 5 to 3 prime
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11
Q

Elongation of transcription

A
  • RNA polymerase travels down DNA template
  • Creates RNA strand with bases complementary to DNA strand
  • RNA strand separates so DNA strand can reattach and recoil
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12
Q

Termination of transcription

A
  • At termination signal polymerase releases DNA and RNA
  • RNA strand that is created is mRNA
  • -goes from the nucleous to the cytosol
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13
Q

what are the 3 types of RNA

A
  • mRNA
  • rRNA
  • tRNA
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14
Q

mRNA

A

messenger RNA

-RNA code for proteins

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15
Q

rRNA

A

Ribosomal RNA

  • RNA-protein complex that reads mRNA and builds proteins
  • in the cytosol mostly on the rough endoplasmic reticulem
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16
Q

tRNA

A

transfer RNA

  • RNA that carries specific amino acids
  • -brings in the necessary amino acids for the rRNA
17
Q

anticodon

A

tRNA nucleotides base trio that corresponds to the specific amino acid called for by the codon
(complementary base pairs for the mRNA)

18
Q

Translation

A

RNA—Protein

  • mRNA carries the code from the nucleous to the cytoplasm
  • ribosomes (rRNA) couple with the mRNA
  • tRNA brings in the appropriate amino acid
    1. Initiation
    2. Elongation
    3. Termination
19
Q

Initiation of translation

A
  • Ribosomes (rRNA) sandwich mRNA

- tRNA attaches to start codon and begins bringing in amino acids

20
Q

Elongation of translation

A

-tRNA keeps shuttling in amino acids one by one

21
Q

Termination of translation

A
  • stop codon reached

- protein and mRNA released

22
Q

Regulation of Genes

A

expression is regulated by differentiaiton and demand

  • can’t make and have the transcription factors for what the cell doesn’t need
  • can be regulated at 4 different steps
23
Q

What are the 4 steps genes can be regulated by?

A
  1. Regulation of transcription
  2. Regulation of translation
  3. Modification/ activation of proteins
  4. Degradation of proteins
24
Q

Regulation of transcription

A

we can activate and deactivate transcription factors as needed

25
Q

Regulation of translation

A

we can control how much gets translated

26
Q

Modification / activation of proteins

A

can control activating proteins and deactivating them

27
Q

Degradation of proteins

A

can break them down when no longer needed