Fou 4 - Cytoplasm and Cytoskeleton Flashcards
What is the function of the Microfilaments?
Muscle contraction and cellular motility. Example: Actin.
What is the function of the intermediate filaments?
Maintain cell structure.
What is the function of the Microtubules?
Movement and cell division.
Which are the immnunohistochemical stains of the intermediate filaments?
Vimentin, Desmin, Cytokeratin, glial fibrillary acid proteins (GFAP), Neurofilaments and Nuclear lamins.
Where can find vimentin?
Connective tissue (fibroblasts, leukocytes, endothelium).
Where can find Desmin?
Muscle cells (smooth, skeletal, heart).
Where can find Cytokeratin ?
Epithelial cells (keratin in desmosomes and hemidesmosomes.
Where can find GFAP?
Astrocytes, Schwann cells, other neuroglia.
Where can find peripherin ?
Neurons.
Where can find Neurofilaments ?
L, M, H molecular weight, Axons within neuron.
Where can find nuclear lamins?
There are A, B and C in the nuclear envelope and DNA within.
Which are the nuclear lamin mutations?
Progeria and muscular dystrophy.
Which cancers are involved to Vimentin?
Sarcomas and some carcinomas.
Which cancer is involved to Desmin?
Myosarcomas; Rabdomyosarcoma and Leiomyosarcoma.
Which cancer is involved to Cytokeratin ?
Carcinomas (skin carcinomas).
Which two cancers are involved to GFAP?
Astrocytoma, Glioblastoma.
Which two cancers are involved to Neurofilaments?
Neuroblastoma and primitive neuroectodemal tumor.
Which are the structures that composed the Microtubules?
Heterodimers alpha and Beta-Tubulin.
Microtubules are incorporated into what three structures?
Flagella, Cilia and Mitotic spindles.
What drugs act on Microtubules?
[Microtubules Get Constructed Very Poorly] Mebendazol, Griseofulvin, Colchicine Vincristine/Vinblastine and paclitaxel and docetaxel.
What does the function of the Molecular motor protein do?
Transport cellular cargo toward opposite ends of Microtubule tracks.
Which are the 2 molecular motor proteins?
Dynein and Kinesin proteins.
Which is the molecular motor protein that go retrograde to Microtubule (+ > -)?
Dynein
Which is the molecular motor protein that go anterograde to Microtubule (- > +)?
Kinesin.
Which are the 3 types of filaments?
Microfilaments. Intermediate filaments. Microtubules.
How is the arrangement of the cilia structure?
9 + 2, Axonemal Dynein.
Which is the type of junction of the cilia structure?
Gap Junction.
Where can we find Cilia?
Respiratory tract (trachea and bronchioles), paranasal sinuses and Fallopian tubes.
What is Kartagener syndrome?
AKA First ciliary dyskinesia, it is a immotile cilia due to a Dynein arm defect.
What are the clinical manifestation of the Kartagener Syndrome?
Infertility (immotile sperm and dysfunctional Fallopian tube cilia), high risk of ectopic pregnancy. Can cause bronchiectasis, recurrent sinusitis, and situs inversus.
What inclusion is caused by Cytomegalovirus?
Owl’s eye inclusions
What inclusion is caused by Rabies virus?
Negri bodies.
What inclusion is caused by Herpes virus?
Cowdry bodies.
What inclusion is found it in Alzheimer disease?
Neurofibrillary tangles.
What are three lysosomal storage disorders?
Gaucherie disease, Niemann-pick disease and Tay-Sachs disease.
What are the two mucopolysaccharidoses?
Hurler syndrome and Hunter syndrome.
What inclusion is found it in Parkinson disease?
Lewy bodies
What intermediate filaments are found in the following tissue types and cellular structures? 1-connective tissue, 2-Muscle tissue, 3-epithelial tissue and 4-Axons
1)Vimentin, 2)Desmin 3)Cytokeratin and 4)Neurofilaments.
What are the defects seen in Kartagener syndrome?
Bronchiectasis, chronic sinusitis, situs inversus and infertility.
