Foty Stem Cell

Question 1

LIF binds to a heterodimer with LIFR and gp130 to activate Jak/stat3 signaling to blocck the ____ Pathway to ____ differentiation

Answer 1

MAPK pathway
Prevents Differentiation

Question 2

Symmetric Stem Cell Division

Answer 2

Giving rise to Two Stem Cells

Question 3

Multi vs Oligopotent Stem Cells

Answer 3

Mutli diffs into lots of closely related cell (hematopoesis)

Oligo can only diff into a few cells (lymp/myeloid progens)

Question 4

Toti vs Pluripotent

Answer 4

• Toti can make all; either embryonic or extra-embryonic tissue (from egg/sperm)
• Pluripotent diff into any of 3 germs (from inner cell mass)

Question 5

Two main characteristics of Stem cells?

Answer 5

1. Unspecialized 2. Self-Renewal

Question 6

3 ways to manipulate stem cell diff

Answer 6

1. Chemical Comp of culture
2. Physical surface of culture
3. Introduce Genes

Question 7

Asymmetric Stem Cell Division

Answer 7

Giving rise to a Stem Cell and Differentiated Cell

Question 8

4 Candidate Genes for Induced Pluripotent Stem Cells

Answer 8

1. Oct4
2. Sox2
3. Klf4
4. cMyc

Question 9

Two Cell-Extrinsic Negative Stem Cell Regulators that discourage differentiation

Answer 9

1. LIF
2. BMP

(Both use STAT3 and SMAD-Id to block MAPK pathway)

Question 10

Four Cell-Intrinsic Negative Stem Cell Regulators that discourage Differentiation

Answer 10

1. Sox2
2. Oct4
3. Nanog
4. Ronin