Apoptosis and Autophagy Flashcards
Name two key Anti-apoptotic proteins
- Bcl-2
- Bfl
“The two hippies Bickle and Biffle”
BH3 Domain Proteins Mnemonic?
“The Noxious Puma Kills the Hippies”
NOX and PUMA stop Bcl and Bfl
What is the histological characteristic of Apoptosis?
Danse, fragmented nucleus, with blebbing on the border
How do you activate a caspase?
Through cleavage, usually by a Cytochrome C enzyme
What two proteins show convergence of Autophagy and Apoptosis?
Bcl-2 and Bcl-XL
Name 4 steps to the Apoptotic Extrinsic Pathway
- Death Receptor DR
- FADD Domain
- DISC
- Auto Caspase Cleaveage
How does Terminal Transfers dUTP Nick-End Labeling (TUNEL) work and what does it identify?
It’s good to identify gaps in DNA, it attaches to the gaps and fluoresces.
True or False: Apoptosis has a massive infammatory response
False
Four Stages of Autophagy
- Induction
- Autophago Form
- Autophago Fuse
- Autophago Break
why does the brain need apoptosis?
Because otherwise it grows too much, it needs caspase 9
Name three key Pro-Apoptotic Proteins
- Bak
- Bax
- BH3 proteins (NOXA PUMA)
Why don’t you see “DNA laddering” in Autophagy, but you do see it in Apoptosis?
Because Apoptosis is dependant on Cleavage-dependent Caspases.
- Autophagy doesn’t use caspases, so the banding patterns of the DNA won’t change
After APAF1 is bound by Cytochrome C, the _____ domains form a cluster of 7 to create the Apoptosome
CARD
Necrosis vs Autophagy appearance
Necrosis’ membrane is complete shredded and vomits everything out
Apop and Autophag has low inflamm
The key player in autophagy, mTor needs to be ____ to lead to dephosphoyrlation events that promote Autophagy
Inactivated
