Forensic Quiz 2 Flashcards
blood
a solution of various materials in water
plasma
liquid portion of blood,55% f total volume
-90% water
10% substances dissolved in it protiens glucose fats hormones nutrients:salt and mineral,antibodies
cells
sold portion of blood,45% of total blood volume
-red blood cells
red blood cells
(RBC,erythrocytes),
-produced in bone marrow
-transport of gases(O2/CO2)
-have no cell nucleus/no nuclear DNA
-hemoglobin-heme unit
-life spans of 120 days
white blood cells
-produced in lymph nodes
-immune response
-nuclear DNA b/c cell nucleus
-life span of 3-20 days
platelets(thromobocytes)
-blood clotting mechanism
-life span of 5-9 days
irregularly shaped
Blood Type A
Antigens: A
Antibodies:B
Donate Blood to:A,AB
Receive blood from:A,O
Blood Type B
Antigens:B
Antibodies:A
Donate Blood to:B,AB
Receive Blood from:B,O
Blood Type AB
Antigens:A,B
Antibodies:None
Donate Blood to:AB
Recieve Blood from:All(universal recipitent)
Blood Type O
Antigens:None
Antibodies:A,B
Donate blood to:All (universal donor)
Receive Blood from:O
RH Factor
another antigen for blood typing
rh+
presence of RH factor
rh-
absence of RH factor
Luminol Reagent(preliminary test for blood)
It reacts with the heme group found in hemoglobin. H₂O₂ (Hydrogen Peroxide): This is a reactive substance that, when added to the reaction, participates in the chemiluminescent process. Heme Catalyst: Heme is a component of hemoglobin (found in red blood cells) and acts as a catalyst in this reaction. It helps to speed up the reaction between luminol and hydrogen peroxide.
-When luminol reacts with hydrogen peroxide in the presence of the heme catalyst, it converts into a product called 3-aminophthalate. This product is key in producing light.
Chemiluminescence: This is the process by which light is emitted as a result of a chemical reaction. In this case, the reaction generates light that lasts about 30 seconds.
No Thickening Agent
Fluorescein-reagent(preliminary test for blood)
hydrogen peroxide is used here as a reactive agent.
Heme Catalyst: The heme group from hemoglobin acts as a catalyst in this reaction,
Shine Short Wave of Light: When a short wave of light is applied to the fluorescein solution, it excites the molecules.
Fluorescent Product: The reaction produces a fluorescent compound that emits light when excited by the short wave of light. This means that, under the right conditions, the sample will glow brightly, indicating the presence of blood.
Fluorescent Reaction: This refers to the process where the fluorescein absorbs light and then re-emits it, creating a visible glow.
Thickening Agent
Confirmatory Test for Blood-Teichmann
Crystalizing regeant+blood(heme) creating microcrystrals
Confirmatory Test for Blood-Species Determination
using immunoprecipitation typing involves mixing rabbit antibodies with human blood serum(antigens). If the antibodies bind to the human antigens in the serum, this indicates that the blood sample is indeed human.
Geometry of a blood stain(surface texture)
hard, non porous surface(glass or smooth tiles) means less splatter
rough surface(concrete/wood) means irregular shaped stain with serrated edges