Food tests: Test for Glucose Flashcards
What do you use?
Benedicts solution
What should happen
Clear blue benedicts solution should turn to brick red precipitate on boiling in the presence of sugar
Never heat with a direct flame. ALWAYS WATER BATH
Never heat with a direct flame. ALWAYS WATER BATH
Method:
- A food sample is mixed with water so that it dissolves
- Several drops of clear blue Benedicts solution are added to a test tube containing the liquid sample
- Place the tube in a water bath
- The two are heated until the water boils
- After a minute, copper 1 oxide precipitate begins to form
Many simple and double sugars (maltose and lactose) are reduce sugars, but sucrose is a non-reducing sugar which will give a negative result when tested this way.
Many simple and double sugars (maltose and lactose) are reduce sugars, but sucrose is a non-reducing sugar which will give a negative result when tested this way.
Explanation:
Glucose is a reducing sugar, because it reduces alkaline blue Copper 2 sulphate in B S into a brick-red precipitate of copper 1 oxide