Final Exam - Recombinant DNA technology

Question 1

Northern analysis

Answer 1

• probes include labeled DNA w/size-fractionated RNA
• used to determine RNA levels for a particular gene at different stages or in different tissues or organs of an organism

Question 2

Restriction Fragment Length Polymorphism (RFLP)

Answer 2

• used to ID differences in restriction pattern of a specific gene or DNA region between several individuals of a species or related species
• aka “DNA finger printing”

Question 3

Polymerase Chain Reaction (PCR)

Answer 3

• used to amplify large amounts of DNA from small samples
• used in crime scenes to ID a suspect
• in vitro DNA synthesis reaction

Question 4

DNA sequencing

Answer 4

• in vitro DNA synthesis using a DNA template, specific primer, dNTPs and DNA polymerase to extend the new strand of DNA
• sequence is determined by fluorescent label attached to dideoxy nucleotide

Question 5

radio labeling a probe

Answer 5

• label DNA w/a radioisotope or fluorescent label that’s detected using x-ray film
• 35S and 32P are isotopes used to label

Question 6

Techniques to isolate DNA

Answer 6

1. gel electrophoresis
2. restriction fragment length polymorphism (RFLP)
3. radio labeling DNA fragments
4. DNA sequencing
5. polymerase chain reaction (PCR)

Question 7

agarose gel electrophoresis

Answer 7

used to fractionate DNA or RNA

Question 8

polyacrylamide gel electrophoresis

Answer 8

used to fractionate proteins and DNA