FDS: spectroscopy UV Vis

Question 1

OUTLINE

Answer 1

Question 2

What if the analytical signal is “light”?

Answer 2

Question 3

Applications in spectroscopy are all based on what?

Answer 3

Question 4

Quantum principles and spectroscopy For polyatomic molecules:

Answer 4

Question 5

For polyatomic molecules: molecular orbitals are involved Transitions:

Answer 5

Question 6

Quantum principles and spectroscopy

delta E?

Answer 6

Question 7

Quantum principles and spectroscopy

delta E based on the structure?

Answer 7

Question 8

Quantum principles and spectroscopy For polyatomic molecules:

E total?

Answer 8

Question 9

Visible and ultraviolet molecular absorption spectra consists of hundreds or thousand of what?

Answer 9

Question 10

Visible light wavelength range

Answer 10

Question 11

Dependence on the nature of the species

Answer 11

Question 12

Magnitude of the absorption of radiation

transmittance

Answer 12

Question 13

Magnitude of the absorption of radiation (Lambert-)Beer’s Law

Answer 13

Question 14

(Lambert-)Beer’s Law Molar absorptivity coefficient ε

Answer 14

Question 15

(Lambert-)Beer’s Law: Deviations

Answer 15

Question 16

Deviations real factors

Answer 16

Question 17

Deviations Instrumental factors

possible source

Answer 17

Question 18

Deviations Instrumental factors • Polychromatic radiation

Answer 18

Question 19

Deviations Instrumental factors • Slit width

Answer 19

Question 20

Deviations Instrumental factors • Stray light

Answer 20

Question 21

Deviations Chemical factors

Answer 21

Question 22

Spectrophotometers Instrumentation Single versus double beam spectrophotometers

Answer 22

Question 23

Sources (line and continuum)

Answer 23

Question 24

Detectors

Answer 24

Question 25

Errors (instrumental)

Answer 25

Question 26

Errors (instrumental)

where is the optimum?

Answer 26

Question 27

Solvents

Answer 27

Question 28

Suppose the question is :

Answer 28

Question 29

Quantification of NH4 +

general

Answer 29

Question 30

Quantification of NH4 +

conversion

Answer 30

Question 31

Quantification of NH4 + Examples Hypochlorous acid (HOCl)

Answer 31

Question 32

Quantification of NH4 +

what is formed?

Answer 32

Question 33

Quantification of NH4 + Examples Finally the oxidized species forms what?

Answer 33

Question 34

Quantification of NH4 +

sample prep

Answer 34

Question 35

Quantification of NH4 + Examples UV/VIS procedure:

Answer 35

Question 36

Quantification of NH4 + Examples Specificity

Answer 36

Question 37

Quantification of NH4 + Examples Robustness of the method

Answer 37

Question 38

Quantification of NH4 +

method validation

Answer 38

Question 39

Suppose the question is :

H2O2

Answer 39

Question 40

Quantification of H2O2

general

Answer 40

Question 41

Quantification of H2O2

problem

Answer 41

Question 42

Quantification of H2O2

linearity

Answer 42

Question 43

Quantification of H2O2

result summary

Answer 43