Factors that affect enzymes Flashcards

And a little about enzymes

1
Q

What is optimal TempreaturE?

Tallk about reaction rate

A

The tempreature which an enzyme works the fastest in, which increases reaction rate.

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2
Q

What happens past optimal tempreture? or pH optimal

A

Denatures, where Hydrogen bonds break down to change the active site of the enzyme, which means the substrate cant bind.

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3
Q

What will happen if substrates increases

A

The rate of reaction increases because there will be more chances of binding

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4
Q

What will happen if you increase enzyme concentation

A

The rate of reaction will increase

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5
Q

What is Saturation point

A

It is when all active sites are used up or all the substrates are have been used up.

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6
Q

NOT RN

What is an competitive Inhibitor

A

They are an molecule that binds to the active site and prevents the substrates from binding.

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7
Q

not now

What is an non-competitive inhibitor

And where does it bind and the effect

A

They dont bind to the active site, instead bind to allohesteric site and changes the enzymes active sites shape

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8
Q

not now

What are reversible inhibitors?

A

They form a weak bond wit the active sites, it can be broken off

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9
Q

not now

What are irreverisble inhibitors

A

They form strong covalent bonds that cant break off.

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10
Q

not now

What are cofactors

A

They help assist the enzymes function by sitting on the active site of the substrate.

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11
Q

nlt now

Is Coenzyme an organic or inorganic cofactor?

A

Organic

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12
Q

not now

What is an coenzyme’s function

AND bro wjat happens after the substrate has been catalysed?

A

It binds and donates energy or molecules

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13
Q

What are enzymes?

A

Enzyme are bilological catalists that speed up chemical reactions with a substrate by lowering the activation energy needed.

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14
Q

What are substrates?

A

The moleucle being acted upon by an enzyme

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15
Q

How do chemical reactions occur?

A

They require input energy called ACTIVATION ENERGY

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16
Q

What are anabolic reactions

A

When two or more smaller moleucles are combined to form a larger moleucle using energy

17
Q

What are catabolic reactions

A

When a large molecule is brken down to two or more smaller molecules

18
Q

What is endergonic and what kind of reaction is it from

A

It means it stores energy, which happens in anabolic reactions

19
Q

What is exergonic and what kind of reaction is it from

A

It means it releases energy from a catabolic reaction

20
Q

What happens to an enzyme after catalysing a reaction?

A

It is reused/recycled

21
Q

What is unique to each enzyme

A

Active site

22
Q

Can enzymes have more than one active site?

23
Q

What do you call it when the substrate and the enzyme has binded

A

Enzyme-substrate complex

24
Q

E

What do you call the end product after the enzyme has sucessfuly catalysed something?

25
Why does the substrate even bind to the active site?
Bercause the substrate is complementary in shape to the active site which is specific.
26
Are enzymes used up or not?
NO
27
What will happen if the pH level of an enzyme gets more basic | and why is it bad
Amino acids will lose their hydrogen ions, which might change their attraction to each other and unfold causing them to denature
28
What will happen if the pH level of an enzyme gets more acidic | and why is it bad
Amino acids will gain hydrogen ions,which might change their attraction to each other and unfold causing them to denature
29
What happens if all the active sites of enzymes have been used up, should you even increase substrate concentration?
No , the substrate concentration has reached a **saturation point**
30
what is a limiting factor when a substrate reaches its saturation point
The lack of Enzyme active sites
31
What ahppens if the tempreture is lowered to an enzyme
There will very low kinetic energy making the enyme work at a very slow rate. This causes less products to be formed due to a lower reaction rate.
32
What type of protiens are enzymes?
Globular protiens
33