Factors that affect enzymes Flashcards

And a little about enzymes

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1
Q

What is optimal TempreaturE?

Tallk about reaction rate

A

The tempreature which an enzyme works the fastest in, which increases reaction rate.

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2
Q

What happens past optimal tempreture? or pH optimal

A

Denatures, where Hydrogen bonds break down to change the active site of the enzyme, which means the substrate cant bind.

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3
Q

What will happen if substrates increases

A

The rate of reaction increases because there will be more chances of binding

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4
Q

What will happen if you increase enzyme concentation

A

The rate of reaction will increase

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5
Q

What is Saturation point

A

It is when all active sites are used up or all the substrates are have been used up.

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6
Q

NOT RN

What is an competitive Inhibitor

A

They are an molecule that binds to the active site and prevents the substrates from binding.

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7
Q

not now

What is an non-competitive inhibitor

And where does it bind and the effect

A

They dont bind to the active site, instead bind to allohesteric site and changes the enzymes active sites shape

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8
Q

not now

What are reversible inhibitors?

A

They form a weak bond wit the active sites, it can be broken off

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9
Q

not now

What are irreverisble inhibitors

A

They form strong covalent bonds that cant break off.

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10
Q

not now

What are cofactors

A

They help assist the enzymes function by sitting on the active site of the substrate.

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11
Q

nlt now

Is Coenzyme an organic or inorganic cofactor?

A

Organic

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12
Q

not now

What is an coenzyme’s function

AND bro wjat happens after the substrate has been catalysed?

A

It binds and donates energy or molecules

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13
Q

What are enzymes?

A

Enzyme are bilological catalists that speed up chemical reactions with a substrate by lowering the activation energy needed.

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14
Q

What are substrates?

A

The moleucle being acted upon by an enzyme

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15
Q

How do chemical reactions occur?

A

They require input energy called ACTIVATION ENERGY

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16
Q

What are anabolic reactions

A

When two or more smaller moleucles are combined to form a larger moleucle using energy

17
Q

What are catabolic reactions

A

When a large molecule is brken down to two or more smaller molecules

18
Q

What is endergonic and what kind of reaction is it from

A

It means it stores energy, which happens in anabolic reactions

19
Q

What is exergonic and what kind of reaction is it from

A

It means it releases energy from a catabolic reaction

20
Q

What happens to an enzyme after catalysing a reaction?

A

It is reused/recycled

21
Q

What is unique to each enzyme

A

Active site

22
Q

Can enzymes have more than one active site?

A

YES

23
Q

What do you call it when the substrate and the enzyme has binded

A

Enzyme-substrate complex

24
Q

E

What do you call the end product after the enzyme has sucessfuly catalysed something?

A

Products

25
Q

Why does the substrate even bind to the active site?

A

Bercause the substrate is complementary in shape to the active site which is specific.

26
Q

Are enzymes used up or not?

A

NO

27
Q

What will happen if the pH level of an enzyme gets more basic

and why is it bad

A

Amino acids will lose their hydrogen ions, which might change their attraction to each other and unfold causing them to denature

28
Q

What will happen if the pH level of an enzyme gets more acidic

and why is it bad

A

Amino acids will gain hydrogen ions,which might change their attraction to each other and unfold causing them to denature

29
Q

What happens if all the active sites of enzymes have been used up, should you even increase substrate concentration?

A

No , the substrate concentration has reached a saturation point

30
Q

what is a limiting factor when a substrate reaches its saturation point

A

The lack of Enzyme active sites

31
Q

What ahppens if the tempreture is lowered to an enzyme

A

There will very low kinetic energy making the enyme work at a very slow rate. This causes less products to be formed due to a lower reaction rate.

32
Q

What type of protiens are enzymes?

A

Globular protiens

33
Q
A