CRISPR CAS-9 Flashcards

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1
Q

Where is CRISPR CAS-9 system found in?

A

In prokaryotes

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2
Q

What is the CRISPR CAS-9 system

A

It is a adaptive immune system that learns previous infections from the cell and develop a memory to slice up the same infection.

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3
Q

What is a Crispr Array system

A

A whole series of spacers

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4
Q

What is the PAM sequence

A

Any nucleotide , folowed by G G
(Any, GG)

Protospacer Adjacent Motif

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5
Q

What does CAS1 and CAS2 do?

A

They read the PAM sequence and then cut out a protospacer to be added to the CRISPR ARRAY

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6
Q

What is unique about the repeats?

A

They are palindromic

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7
Q

What does RNAase do?

A

Transcribes pre-crRNA

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8
Q

Where does unprocessed tracrRNA bind to?

A

Repeat reigions

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9
Q

What is cr:tracrRNA reffered to as when binded to CAS-9 protien

A

gRNA

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10
Q

What does Cas-9 protien do

A

It binds to PAM sequence, and checks if protospacer is complementary to the gRNA then cuts to disable virus.

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11
Q

what is sgRNA

A

single Guide RNA, made by scientists to be used on CAS-9 protien for own applications

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12
Q
A
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