experiemental techniques Flashcards
1
Q
describe in situ hybridisation
A
target = DNA/RNA
fixed cells/tissues then add complementary probe with nucleic acids
used to see where a specific gene is being transcribed/expressed
2
Q
describe GFP
A
gene editing to introduce GFP to proteins of interest
tracks live gene activity so studying protein-protein interactions
3
Q
describe immunohistochemistry
A
target= proteins
fixed cells/tissue
complementary antibodies added, usually with fluorescent marker
