Exercise No. 6c-1 PROTOZOA Phylum Sarcomastigophora - Subphylum Mastigophora Flashcards

1
Q

Trophozoite: 9 to 12 µm long by 5 to l5 µm wide

A

Giardia lamblia

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2
Q

Trophozoite: average size of 14 x 7 µm

A

Giardia lamblia

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3
Q

Trophozoite: pyriform, pear-shaped or teardrop shaped

A

Giardia lamblia

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4
Q

Trophozoite: rounded anterior

A

Giardia lamblia

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5
Q

Trophozoite: tapering, pointed, attenuated posterior

A

Giardia lamblia

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6
Q

Trophozoite: resembles the curved portion of a spoon if examined from the side

A

Giardia lamblia

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7
Q

Trophozoite: dorsoventrally flattened

A

Giardia lamblia

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8
Q

Trophozoite: dorsal side is convex and the ventral side is flat or concave

A

Giardia lamblia

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9
Q

Trophozoite: anterior half of the ventral surface bears two sucking discs on each side

A

Giardia lamblia

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10
Q

Trophozoite: bilaterally symmetrical with a pair of medial lines

A

Giardia lamblia

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11
Q

Trophozoite: possesses two ovoid to spherical nuclei, located one in each of the sucking disc

A

Giardia lamblia

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12
Q

Trophozoite: Each nucleus has a large karyosome, usually centrally located

A

Giardia lamblia

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13
Q

Trophozoite: no peripheral chromatin

A

Giardia lamblia

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14
Q

Trophozoite: Two slightly curved rodlike structures sit on the axonemes posterior to the nuclei

A

Giardia lamblia

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15
Q

Trophozoite: four pairs of flagella that arise from the blepharoplasts

A

Giardia lamblia

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16
Q

Trophozoite: One pair of the flagella projects from the the anterior end, another pair on the posterior end, and 2 pairs on the midportion extending laterally.

A

Giardia lamblia

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17
Q

Trophozoite: propelled into a “falling leat” or “flipflop” motility

A

Giardia lamblia

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18
Q

Trophozoite: “monkey face”

A

Giardia lamblia

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19
Q

Trophozoite: “old man with eyeglasses”

A

Giardia lamblia

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20
Q

used for attachment to the intestinal mucosa

A

sucking discs

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21
Q

bilaterally symmetrical with a pair of medial lines dividing the trophozoite into 2 halves throughout most of its length

A

axostyles (or axonemes)

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22
Q

Two slightly curved rodlike structures

A

parabasal bodies (or median bodies)

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23
Q

Cyst: ovoid or ellipsoidal in shape

A

Giardia lamblia

24
Q

Cyst: 8 to 12 µm long by 7 to 10 µm wide

A

Giardia lamblia

25
Q

Cyst: surrounded by a tough hyaline cyst wall secreted from cytoplasm

A

Giardia lamblia

26
Q

Cyst: cytoplasm may retract from the cyst wall creating a clearing zone and a double wall appearance

A

Giardia lamblia

27
Q

Cyst: immature cyst contains two nuclei

A

Giardia lamblia

28
Q

Cyst: mature cyst has four

A

Giardia lamblia

29
Q

Cyst: axostyles lie diagonally forming a dividing line

A

Giardia lamblia

30
Q

Cyst: Remnants of the flagella and parabasal bodies are seen

A

Giardia lamblia

31
Q

Trophozoite: 5- 12 µm in diameter

A

Dientamoeba fragilis

32
Q

Trophozoite: slightly progressive motility

A

Dientamoeba fragilis

33
Q

Trophozoite: does not have flagella

A

Dientamoeba fragilis

34
Q

Trophozoite: characterized as having one (20 to 40%) or two (60 to 80%) nuclei

A

Dientamoeba fragilis

35
Q

Trophozoite: nuclear chromatin is usually fragmented into three to five granules

A

Dientamoeba fragilis

36
Q

Trophozoite: no peripheral chromatin on the nuclear membrane

A

Dientamoeba fragilis

37
Q

Trophozoite: has a hyaline ectoplasm

A

Dientamoeba fragilis

38
Q

Trophozoite: highly vacuolated endoplasm with ingested bacteria, yeast, and other debri

A

Dientamoeba fragilis

39
Q

possess characteristic serrate margins which sometimes give it a stellate appearance

A

broad hyaline pseudopodia

40
Q

Giardiasis can be diagnosed by identification of cysts or trophozoites of Giardia lamblia in stool by

A

direct fecal smears, concentration, or permanent staining

41
Q

Cysts of G. lamblia are found in

A

formed stools

42
Q

Cysts and trophozoites of G. lamblia are found in

A

diarrheal stools

43
Q

Often multiple specimens need to be examined and concentration techniques prior to reporting that a patient is free of Giardia.

A

Direct Microscopy: Stool examination

44
Q

This is done if the stool exam reveals negative results despite the clinical manifestations of giardiasis.

A

Examination of duodenal specimen

45
Q

Obtained by gastroduodenoscopy and examined microscopically as a wet preparation or permanent-stained smear

A

Duodenal aspirate

46
Q

another method for obtaining duodenal specimen

A

Enterotest® (HDC Mountain View, CA) or string test

47
Q

a gelatin capsule lined with silicone rubber that contains a spool of nylon string and a weight

A

Enterotest® (HDC Mountain View, CA) or string test

48
Q

The end of the string is taped to the back of the patient’s [?]

A

neck or cheek

49
Q

The patient then swallows the [?]

A

capsule with water

50
Q

The patient is not allowed to eat during this time but is allowed to [?]

A

drink a small amount of water

51
Q

As the capsule dissolves, the string unwinds and is carried by [?] to the duodenum, and the duodenal mucus adheres to the string.

A

peristalsis

52
Q

After about [?], the string is retrieved, and duodenal contents adherent to the string is scraped off and placed in saline on the slide for microscopic examination.

A

4 to 6 hours

53
Q

also used for detecting larvae of Strongyloides, eggs of liver flukes and oocysts of lsospora

A

Enterotest® (HDC Mountain View, CA) or string test

54
Q

A variety of immunodiagnostic methods, relying on detection of serum antibodies or antigens in feces, are in use.

A

Immunodiagnosis of Giardia lamblia

55
Q

have been used to demonstrate parasitic genome in the stool specimen containing Giardia lamblia

A

Deoxyribonucleic acid (DNA) probes and polymerase chain reaction (PCR)

56
Q

has only the trophozoite stage; Cysts have not been identified

A

Dientamoeba fragilis