Exercise 5: Microscopy Flashcards

1
Q

Condenser

A

collect the light rays emitted by its source and direct it to the specimen

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2
Q

Numerical Aperture

A

a measure of its ability to gather light and resolve details of the specimen at a fixed length

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3
Q

Limit of resolution (D)

A

a microscope is the minimum distance between two objects where they can be seen as two separate entities
-short wave lengths filters can decrease D.

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4
Q

Total Magnification

A

calculated by multiplying the power of the OBJECTIVE LENS x power of the OCULAR LENS (10x)

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5
Q

Bright-Field Microscopy

A
  • visible light is used
  • contrast between the specimen and the background enables the clarity of the picture
  • staining improves contrast
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6
Q

Phase-Contrast Microscopy

A
  • designed to create a phase shift in light

- useful for live specimens because staining is not necessary

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7
Q

Dark-Field Microscopy

A
  • scattered light from the specimen reaches the objective lens forming images of light objects in dark backgrounds
  • resolution is better
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8
Q

Fluorescence Microscopy

A
  • fluorescent dyes are used

- dyes absorb light of one wavelength and emit light at another wavelength

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9
Q

Differential Interference Contrast (DIC) Microscopy

A
  • works best for live specimens because this type of microscope allows the clear visualization of the internal structures
  • does not require staining
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