Exercise 3: Aseptic Transfer & Inoculation Methods Flashcards
1
Q
Bacterial culture
A
bacteria and the medium it is growing in
-ex: E. coli growing in TSB
2
Q
Pure culture
A
contains a single type of microbe
3
Q
Mixed culture
A
contains two or more types of microbes
4
Q
Growth Cycle
A
lag phase, exponential phase, stationary phase, and death
5
Q
Lag phase
A
a period of resting when bacterium is introduced into a new culture medium
- bacteria are preparing to grow in this phase
- this depends on growth medium and culture age
6
Q
Exponential phase or log phase
A
the healthiest growth phase of the bacterium, the growth of a bacterium is exponential
7
Q
Stationary phase
A
the end of the bacterium’s growth cycle
8
Q
Death phase
A
cells begin to lyse
9
Q
Sub-culturing (culture transferring)
A
necessary to maintain bacteria in viable cultures for the purpose of research and lab work
- cells from one culture is transferred to a fresh medium in order to maintain the viability of the bacterium of interest
- ASEPTIC TECHNIQUE required
