Exercise 4: Streak Plate Method of Isolation

Question 1

Streak Plate Method of Isolation

Answer 1

the goal is to achieve isolated colonies or colony forming units
-ASEPTIC TECHNIQUE required

Question 2

Colony

Answer 2

a visible mass of cells on a petri dish arising from a single cell
-will have genetically identical cells since they originate from a single cell

Question 3

Dilution gradient

Answer 3

taking from high concentration to low concentration; carries fewer and fewer cells into the subsequent areas of streaking

Question 4

Colony Characteristics

Answer 4

shape (form), margin, elevation, texture, surface, color

Question 5

Shape

Answer 5

circular, irregular, punctiform (tiny)

Question 6

Margin

Answer 6

entire (smooth), undulate (wavy), lobate (lobed), filamentous or rhizoid

Question 7

Elevation

Answer 7

flat, raised, convex, pulvinate (very convex) and umbonate (raised center)

Question 8

Texture

Answer 8

moist, mucoid/slimy, dry

Question 9

Surface

Answer 9

rough, smooth, glistening, wrinkled

Question 10

Color

Answer 10

may be combined with optical properties such as opaque, translucent, shiny or dull