Exam Stuff Flashcards
The secretory component of IgA dimers serves the following function
Protects the dimers from proteolytic digestion
Which of the following cell typed utilize constant fragment recepetors FcR?
All of the above
Neutrophils
Macrophages
NK cells
(B,T, Mast cells)
The antibodies with a 4th CH domain (CH4) are
IgM,IgE
An anti-isotypic antibody can be generated by injected a human antibody into a mouse.
True
Allotypic antibodies arise from small differences of amino acid sequences between two different classes of antibodies
True
Idiotypic antibodies can be generated between 2 genetically identical individuals
False
Monoclonal antibodies can be used in diagnostic imaging
True
Indirect ELISA requires two antibodies capable of binding the molecules that is being assayed
True
Sandwich ELISA makes use of a capture and detection antibody.
True
Adhering of capture antibody in a sandwich ELiSA is acheived by incubating the antibody in the plate overnight at 37 celsius.
False (4 celsius is correct answer)
The blocking step minimizes non-specific binding of the capture antibody on the plate.
True
10% FBS is a good option for carrying out the blocking step of an ELISA
True
Which of the following are characteristics of NK cells.
A and B
A. Eliminate virally infected cells
B. Express CD 16 marker
Antibody dependent cell cytotoxicity (ADCC) requires
A and B
A. An antibody against the tumor (antibody dependent, duh)
B. The CD16 receptor
The regression value (R2) is used to determine how good a standard curve is at
Above 0.9
