Exam Flashcards
1
Q
In a sandwich ELISA the detection antibody
A
Is conjugated to biotin
2
Q
The “blocking” step in ELISA is needed to
A
Avoid non-specific binding
3
Q
The avidin protein has high affinity for
A
None of the above (biotin)
4
Q
The avidin protein is conjugated to the
A
HRP enzyme
5
Q
It is necessary to wash thoroughly after addition of the avidin-HRP
A
To minimize non-specific
avidin-HRP binding
6
Q
Standards are set up by using
A
Recombinant cytokine of known concentration
