Enzymatic digestion Of Antibodies Flashcards
1
Q
Fab stands for
A
Fragment that is Antigen Binding
2
Q
Digestion with Papain yields
A
3 Fragments
2 identical Fab+ 1Fc
Fc b/c crystallizes in cold storage
3
Q
Pepsin Digestion results in
A
2 Fab’s
No Fc recovery , it was digested entirely
4
Q
Mecaptoethanol reduction
A
Eliminates disulfide bonds makes them into sulfahydrals
