Antibody Structure Part 2

Question 1

Papain digestion

Answer 1

Cleaves at the hinge (black part) which separtes both fabs

Question 2

Mercaptoethanol

Answer 2

Reducing agent

Reduces the disulfide bonds to sulfahydrides

Question 3

Pepsin Digestion

Answer 3

Cleaves at the FC region leaving it in fragment soo the two fabs are still connected together by the hinge

Question 4

Repeating domains

Answer 4

110a/a

Question 5

Intrachain disulfide bonds

Answer 5

Within the domain (inside any variable or constant)

Question 6

Interdisulfide

Answer 6

In the hinge

Question 7

Heavy can have 1V and about _______ constants?

Answer 7

3-4

Question 8

Lights chains will always have 1 variable and ____ constant.

Answer 8

1

Question 9

Hinge Region is rich in

Answer 9

proline residues (flexible)

Question 10

Hinge is found in

Answer 10

IgG
IgA
IgD

Question 11

Hinge region: Proline residues are target for

Answer 11

Proteolytic digestion (papain and pepsin)

Question 12

Hinge regin has disulfie bonds which means its rich in

Answer 12

Cysteine residues

Question 13

Which immunoglobun classes don’t have a hinge region?

Answer 13

IgM
IgE

INSTEAD they have an EXTRA Constant Heavy domain making 4 of them instead of just 3 domains