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Bacteriology > Exam 8 > Flashcards

Exam 8 Flashcards

1
Q

What is the gram stain morphology of pasteurella
What biochemicals is it positive for

A

small GNR,
non motile
glucose fermenting
oxidase positive

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2
Q

What media does pasteurella grow on

A

Chocolate and BAP
not MAC

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3
Q

What is the epidemiology for pasteurella

A

zoonotic
normal flora in domestic and wild animals

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4
Q

What bacteria is associated with bite wounds from dogs or cats

A

Pasteurella

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5
Q

What is the most relevant species in pasteurella

A

P. multicoda

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6
Q

What are the results for Pasteurella
oxidase
nitrate
ornithine
indole
urease

A

oxidase pos
nitrate pos
orni pos
indole neg
urease neg

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7
Q

What is the plate morphology of pasteurella

A

gamma/ non hemolytic
musty smell
mucoid
no growth on MAC

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8
Q

What antibiotics are useful for pasteurella multocida

A

penicillin

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9
Q

How can you distinguish Pasteurella and Hemophilus

A

haemophilus do not grow on BAP

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10
Q

What is the gram stain morphology for Bartonella

A

short Gram neg pleomorphic bacillus

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11
Q

What agar does Bartonella grow on

A

BAP and CHOC not MAC

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12
Q

What are the results for Bartonella
oxidase
catalase

A

oxidase neg
catalase neg

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13
Q

Organism that can be acquired naturally or through anthroponotic/ human reservior

A

Bartonella

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14
Q

Match the species to pathogenesis
B. quintana
B. bacilliformis
B. Henselae

Cat scratch fever
Oroya fever
Trench fever

A

B. quintana- trench fever
B. bacilliformis- Oroya fever
B. henselae- cat scratch fever

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15
Q

What organism is capable of causing angiogenesis and define it.

A

Bartonella species, mimics cancer in the formation of new blood vessels

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16
Q

What disease profile is this: progressive febrile anemia, verrugas on body

A

Oroya fever

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17
Q

What disease profile is this
S&S like mild influenza, maculopapular rash, bone pain

A

Trench fever

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18
Q

What special plates do Bartonella grow on

A

horse or rabbit blood agar

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19
Q

Organism detected with 16S RNA, or genes gltA, groEL, ribC, ftsZ

A

Bartonella

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20
Q

Match vector to organism: B. henselae, B, quintana
cat flea
human louse

A

B.henselae cat flea
B. quintana human louse

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21
Q

What is the gram stain morphology of helicobacter

A

curved, helical or SPIRAL, fusiform pointed, GNR,

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22
Q

What biochemicals is helicobacter positive for

A

urease strong pos
oxidase pos
catalase pos

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23
Q

What organism is strongly related to stomach cancer

A

helicobacter

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24
Q

How is helicobacter transmitted ?

A

fecal-oral and environmental

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25
Q

How does helicobacter attack the stomach

A

escapes the acidity of the stomach, burrows it and colonizes the gastric mucosa, it neutralizes the stomach pH

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26
Q

What organism infects patients and develops peptic ulcer disease and gastric carcinoma

A

H. pylori

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27
Q

What organism produces a protein ____ that enables it to invade the gastric epithelium

A

H. pylori
CagA

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28
Q

T or F H. pylori can be cultured with fecal specimens

A

false, they can only be used for antigen tests

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29
Q

How can H. pylori be detected with nucleic acid detection

A

gastric acid can be tested

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30
Q

How can you culture H. pylori

A

CHOC agar
must be fresh and moist, needs high humidity

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31
Q

What organism can be IDed with a urea breath test

A

H. pylori

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32
Q

How can you distinguish H.pylori from vibrio and other gastrointestinal organisms

A

H. pylori does not cause diarrhea

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33
Q

What genes are used to ID helicobacter in PCR

A

ureA and cagA

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34
Q

What is used to detect helicobacter directly

A

with a special spectrometer

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35
Q

How is helicobacter detected in serology testing

A

J chain on IgA in stomach acids

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36
Q

How to distinguish Helicobacter and Campy

A

helicobacter- stomach and duodenum
campy-small and large intestine

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37
Q

What is the most common cause of diarrhea in the US and worldwide due to its low ID___

A

campy
id50

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38
Q

How is campy transmitted

A

by eating raw or **undercooked poultry **and seafood.

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39
Q

What kind of growth requirements do Campy have

A

slow growing, fastidious, microaerophillic, needs 5-10% O2

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40
Q

What are the 3 main diseases that campy casues

A

febrile systemic disease
periodontal disease
gastroenteritis

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41
Q

What organism has an S shape or seagull wing

A

Campy

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42
Q

What organism has darting motility

A

Campy

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43
Q

What are the reactions for Campy
oxidase
fermentation

A

oxidase pos
non fermentation

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44
Q

What Campy species is associated with sepsis in the immunocompromised/ elderly

A

C. fetus

45
Q

Organism is transmitted via contaminated food, milk or water and improperly pasteurized milk

A

campy

46
Q

What organism on rare occasion causes Guillain Barre syndrome

A

Campy

47
Q

T or F fecal white cells is a good test for finding Campy infections

A

False, better to take screening tests for inflammation, gram stains on stool is not recommended

48
Q

What organisms can be detected in EIAs

A

Campy

49
Q

How soon should a campy specimen be processed into the lab

A

2 hrs

50
Q

What special agars are used to culture Campy

A

enriched BA
CCDA

51
Q

What is in CCDA

A

instead of blood it has charcoal, sodium pyruvate and ferrous sulfate

52
Q

What kind of samples must be used to culture Campy species

A

must be from watery cultures, never formed stool

53
Q

How much time does it take Campy to grow

A

less than 5 days in routine blood culture media

54
Q

What is the colony morphology of Campy

A

gray-pink or yellow grey mucoid

55
Q

How can you distinguish C. jejuni from C. coli

A

C. jejuni- sodium hippurate hydrolysis +
C. coli neg

56
Q

What campy species is incapable of growth in 42C, instead grows best at 37C-25C

A

C. fetus

57
Q

What campy is not enteric

A

C. fetus

58
Q

What are the results for NA and cephasporin disks for campy species
jejuni, coli, lari and fetus

A

jejuni and coli are sensitive to NA and Resistant to Cephalothin
fetu is R to NA and Sensitive to cephalothin
C. lari is R to both

59
Q

What antibiotics to treat Campy severe gastroenteritis infection

A

erythromycin

60
Q

T or F, there are vaccines available for Campy

A

False, no vaccines

61
Q

All legionella are

A

fastidious, motile, mesophilic, thin GNR

62
Q

What organisms require irone, L cysteine, branched fatty acids and ubiquinones

A

Legionella

63
Q

Organisms that are found in aquatic habitats at warmer temperatures, free flowing water, lakes, rivers

A

Legionella

64
Q

Organism that uses biofilms to survive phagocytosis of monocytes and aveolar macrophages

A

legionella pneumophila

65
Q

What is the main form of transmission of Legionella

A

inhalation of aerosols

66
Q

What body sites can Legionella come from

A

respiratory secretions, sputum, bronchial lavage, lung biopsy

67
Q

What disease profile is this
febrile, cough, headache, pulmonary infiltrates

A

Legionnaire’s disease

68
Q

What media is used to culture legionella

A

BCYE

69
Q

What organism causes pontiac fever

A

legionella

70
Q

Organism that forms gray white colonies like ground glass on BCYE

A

Legionella

71
Q

How can you distinguish L. pneumophila from other Legionella species

A

L. pneumophila does not fluoresce under UV light

72
Q

What is the colony morphology of Legionella

A

Gram negative coccobacili or short rods, weakly staining, cut glass internal granular speckling

73
Q

What are the results for Legionella
oxidase
catalase
Beta lactamase

A

positive for all

74
Q

How can you detect Legionella with Serology testing

A

serum species tested no closer than 2 weeks apart, after the appearance of the disease, antibody testing

75
Q

What type of assay can be used to detect legionella

A

DFA direct fluorescent assay
FITC conjugates

76
Q

What DNA sequences for Legionella are tested

A

mip and wzm genes

77
Q

What antibiotics should be used against Legionella

A

quinolones, tetracycline and macrolides

78
Q

What growth requirements do HACEK organisms have

A

5-10% CO2, will not grow on MAC

79
Q

All HACEK organisms

A

are dysgonic-grow slowly, ferment glucose, are fastidious, are GNR, grow on SBA and CHOC

80
Q

What organisms need a CO2 incubator and candle jar

A

All HACEK organisms

81
Q

HACEK organisms are normal flora of

A

nasopharynx and oral cavity of humans and other animals

82
Q

HACEK that causes bacterial sub acute endocarditis, soft tissue infections

A

A, C and K
aggregatibacter
Cardiobacterium
Kingella

83
Q

HACEK that causes sepsis and endogenous infections of patients with neutropenia

A

Capnocytophaga

84
Q

HACEK that causes DIC, renal failure, shock and HUS from animal bites

A

C. canimorsus and C. cynodegmi

85
Q

What HACEK organisms cause endocarditis from peridontal disease

A

cardiobacterium

86
Q

What HACEK organism causes osteoarthritis in children

A

Kingella

87
Q

How to distinguish Actinobacillus and Aggregatibacter and Pasteurella

A

MALDI-TOF

88
Q

What organism looks like dots and dashes of morse code

A

Actinobacillus

89
Q

What organisms have a 4 to 6 pointed star in colonies

A

Actinobacillus actinomyecetemcomitans

90
Q

Organism in HACEK that causes SBE subacute bacterial endocarditis

A

A. a horrible long name

91
Q

What organism looks like tiny puffballs growing on blood cell layer in SBA

A

Aggregatibacter species

92
Q

What organism can be found in dental plaque and gingival scrapings

A

Aggregatibacter aphrophilus

93
Q

How can you distinguish Aggregatobacter aphrophilus from A. actino-horrible long name

A

actino- catalase pos lactose and indole neg
Aggre- catalase neg lactose and indole pos

94
Q

Organism that pits the agar but has no bleach odor

A

Carbiobacterium

95
Q

Organism that has a gram stain with a teardrop appearance in clusters or rosettes

A

Carbiobacterium hominis

96
Q

What are the results for cardiobacterium
indole
oxidase
catalse

A

indole oxi pos
catalase neg

97
Q

Organism highly recovered in children’s upper respiratory tract

A

Kingella

98
Q

organism that looks like plump coccobacilli with squared off ends

A

Kingella species

99
Q

What media is used to isolate Kingella species

A

MTM

100
Q

How can you differentiate Kingella and Neisseria

A

Neisseria catalase neg
Kingella catalase pos

101
Q

What organisms affect bones and joints in children

A

Kingella kingae

102
Q

HACEK that has gliding motility and is associated with dog bites

A

Capnocytophaga

103
Q

What organism is inhibited by SPS

A

Capnocytophagia

104
Q

What is the only Capnocytophaga species that is both catalase and oxidase pos

A

C. canimorsus

105
Q

yellow-pigmented, thin, gram-negative rod with tapered ends that exhibits gliding motility and does not grow in ambient air

A

Capnocytophaga

106
Q

What organism is encapsulates, plemorphic GNR

A

Klebsiella granulomatis

107
Q

Organism that is inside macrophages or donovan bodies, cause donovanosis

A

Klebsiella granulomatis

108
Q

Organism that can cause genital ulcers and subcutaneous nodules that enlarge

A

Klebsiella

Bacteriology (10 decks)

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