Exam 8 Flashcards
What is the gram stain morphology of pasteurella
What biochemicals is it positive for
small GNR,
non motile
glucose fermenting
oxidase positive
What media does pasteurella grow on
Chocolate and BAP
not MAC
What is the epidemiology for pasteurella
zoonotic
normal flora in domestic and wild animals
What bacteria is associated with bite wounds from dogs or cats
Pasteurella
What is the most relevant species in pasteurella
P. multicoda
What are the results for Pasteurella
oxidase
nitrate
ornithine
indole
urease
oxidase pos
nitrate pos
orni pos
indole neg
urease neg
What is the plate morphology of pasteurella
gamma/ non hemolytic
musty smell
mucoid
no growth on MAC
What antibiotics are useful for pasteurella multocida
penicillin
How can you distinguish Pasteurella and Hemophilus
haemophilus do not grow on BAP
What is the gram stain morphology for Bartonella
short Gram neg pleomorphic bacillus
What agar does Bartonella grow on
BAP and CHOC not MAC
What are the results for Bartonella
oxidase
catalase
oxidase neg
catalase neg
Organism that can be acquired naturally or through anthroponotic/ human reservior
Bartonella
Match the species to pathogenesis
B. quintana
B. bacilliformis
B. Henselae
Cat scratch fever
Oroya fever
Trench fever
B. quintana- trench fever
B. bacilliformis- Oroya fever
B. henselae- cat scratch fever
What organism is capable of causing angiogenesis and define it.
Bartonella species, mimics cancer in the formation of new blood vessels
What disease profile is this: progressive febrile anemia, verrugas on body
Oroya fever
What disease profile is this
S&S like mild influenza, maculopapular rash, bone pain
Trench fever
What special plates do Bartonella grow on
horse or rabbit blood agar
Organism detected with 16S RNA, or genes gltA, groEL, ribC, ftsZ
Bartonella
Match vector to organism: B. henselae, B, quintana
cat flea
human louse
B.henselae cat flea
B. quintana human louse
What is the gram stain morphology of helicobacter
curved, helical or SPIRAL, fusiform pointed, GNR,
What biochemicals is helicobacter positive for
urease strong pos
oxidase pos
catalase pos
What organism is strongly related to stomach cancer
helicobacter
How is helicobacter transmitted ?
fecal-oral and environmental
How does helicobacter attack the stomach
escapes the acidity of the stomach, burrows it and colonizes the gastric mucosa, it neutralizes the stomach pH
What organism infects patients and develops peptic ulcer disease and gastric carcinoma
H. pylori
What organism produces a protein ____ that enables it to invade the gastric epithelium
H. pylori
CagA
T or F H. pylori can be cultured with fecal specimens
false, they can only be used for antigen tests
How can H. pylori be detected with nucleic acid detection
gastric acid can be tested
How can you culture H. pylori
CHOC agar
must be fresh and moist, needs high humidity
What organism can be IDed with a urea breath test
H. pylori
How can you distinguish H.pylori from vibrio and other gastrointestinal organisms
H. pylori does not cause diarrhea
What genes are used to ID helicobacter in PCR
ureA and cagA
What is used to detect helicobacter directly
with a special spectrometer
How is helicobacter detected in serology testing
J chain on IgA in stomach acids
How to distinguish Helicobacter and Campy
helicobacter- stomach and duodenum
campy-small and large intestine
What is the most common cause of diarrhea in the US and worldwide due to its low ID___
campy
id50
How is campy transmitted
by eating raw or **undercooked poultry **and seafood.
What kind of growth requirements do Campy have
slow growing, fastidious, microaerophillic, needs 5-10% O2
What are the 3 main diseases that campy casues
febrile systemic disease
periodontal disease
gastroenteritis
What organism has an S shape or seagull wing
Campy
What organism has darting motility
Campy
What are the reactions for Campy
oxidase
fermentation
oxidase pos
non fermentation
What Campy species is associated with sepsis in the immunocompromised/ elderly
C. fetus
Organism is transmitted via contaminated food, milk or water and improperly pasteurized milk
campy
What organism on rare occasion causes Guillain Barre syndrome
Campy
T or F fecal white cells is a good test for finding Campy infections
False, better to take screening tests for inflammation, gram stains on stool is not recommended
What organisms can be detected in EIAs
Campy
How soon should a campy specimen be processed into the lab
2 hrs
What special agars are used to culture Campy
enriched BA
CCDA
What is in CCDA
instead of blood it has charcoal, sodium pyruvate and ferrous sulfate
What kind of samples must be used to culture Campy species
must be from watery cultures, never formed stool
How much time does it take Campy to grow
less than 5 days in routine blood culture media
What is the colony morphology of Campy
gray-pink or yellow grey mucoid
How can you distinguish C. jejuni from C. coli
C. jejuni- sodium hippurate hydrolysis +
C. coli neg
What campy species is incapable of growth in 42C, instead grows best at 37C-25C
C. fetus
What campy is not enteric
C. fetus
What are the results for NA and cephasporin disks for campy species
jejuni, coli, lari and fetus
jejuni and coli are sensitive to NA and Resistant to Cephalothin
fetu is R to NA and Sensitive to cephalothin
C. lari is R to both
What antibiotics to treat Campy severe gastroenteritis infection
erythromycin
T or F, there are vaccines available for Campy
False, no vaccines
All legionella are
fastidious, motile, mesophilic, thin GNR
What organisms require irone, L cysteine, branched fatty acids and ubiquinones
Legionella
Organisms that are found in aquatic habitats at warmer temperatures, free flowing water, lakes, rivers
Legionella
Organism that uses biofilms to survive phagocytosis of monocytes and aveolar macrophages
legionella pneumophila
What is the main form of transmission of Legionella
inhalation of aerosols
What body sites can Legionella come from
respiratory secretions, sputum, bronchial lavage, lung biopsy
What disease profile is this
febrile, cough, headache, pulmonary infiltrates
Legionnaire’s disease
What media is used to culture legionella
BCYE
What organism causes pontiac fever
legionella
Organism that forms gray white colonies like ground glass on BCYE
Legionella
How can you distinguish L. pneumophila from other Legionella species
L. pneumophila does not fluoresce under UV light
What is the colony morphology of Legionella
Gram negative coccobacili or short rods, weakly staining, cut glass internal granular speckling
What are the results for Legionella
oxidase
catalase
Beta lactamase
positive for all
How can you detect Legionella with Serology testing
serum species tested no closer than 2 weeks apart, after the appearance of the disease, antibody testing
What type of assay can be used to detect legionella
DFA direct fluorescent assay
FITC conjugates
What DNA sequences for Legionella are tested
mip and wzm genes
What antibiotics should be used against Legionella
quinolones, tetracycline and macrolides
What growth requirements do HACEK organisms have
5-10% CO2, will not grow on MAC
All HACEK organisms
are dysgonic-grow slowly, ferment glucose, are fastidious, are GNR, grow on SBA and CHOC
What organisms need a CO2 incubator and candle jar
All HACEK organisms
HACEK organisms are normal flora of
nasopharynx and oral cavity of humans and other animals
HACEK that causes bacterial sub acute endocarditis, soft tissue infections
A, C and K
aggregatibacter
Cardiobacterium
Kingella
HACEK that causes sepsis and endogenous infections of patients with neutropenia
Capnocytophaga
HACEK that causes DIC, renal failure, shock and HUS from animal bites
C. canimorsus and C. cynodegmi
What HACEK organisms cause endocarditis from peridontal disease
cardiobacterium
What HACEK organism causes osteoarthritis in children
Kingella
How to distinguish Actinobacillus and Aggregatibacter and Pasteurella
MALDI-TOF
What organism looks like dots and dashes of morse code
Actinobacillus
What organisms have a 4 to 6 pointed star in colonies
Actinobacillus actinomyecetemcomitans
Organism in HACEK that causes SBE subacute bacterial endocarditis
A. a horrible long name
What organism looks like tiny puffballs growing on blood cell layer in SBA
Aggregatibacter species
What organism can be found in dental plaque and gingival scrapings
Aggregatibacter aphrophilus
How can you distinguish Aggregatobacter aphrophilus from A. actino-horrible long name
actino- catalase pos lactose and indole neg
Aggre- catalase neg lactose and indole pos
Organism that pits the agar but has no bleach odor
Carbiobacterium
Organism that has a gram stain with a teardrop appearance in clusters or rosettes
Carbiobacterium hominis
What are the results for cardiobacterium
indole
oxidase
catalse
indole oxi pos
catalase neg
Organism highly recovered in children’s upper respiratory tract
Kingella
organism that looks like plump coccobacilli with squared off ends
Kingella species
What media is used to isolate Kingella species
MTM
How can you differentiate Kingella and Neisseria
Neisseria catalase neg
Kingella catalase pos
What organisms affect bones and joints in children
Kingella kingae
HACEK that has gliding motility and is associated with dog bites
Capnocytophaga
What organism is inhibited by SPS
Capnocytophagia
What is the only Capnocytophaga species that is both catalase and oxidase pos
C. canimorsus
yellow-pigmented, thin, gram-negative rod with tapered ends that exhibits gliding motility and does not grow in ambient air
Capnocytophaga
What organism is encapsulates, plemorphic GNR
Klebsiella granulomatis
Organism that is inside macrophages or donovan bodies, cause donovanosis
Klebsiella granulomatis
Organism that can cause genital ulcers and subcutaneous nodules that enlarge
Klebsiella