Exam 3 Review

Question 1

False Negative Reactions

Answer 1

Can occur when antigen-antibody reactions have occurred but WASHING IS INADEQUATE & free antibody remains when the AHG is added (inadequate cell washing, delay in adding AHG after washing, presence of small fibrin clots among cells, inactive/forgotten reagent, shaking too hard

Question 2

False Positive Reactions

Answer 2

Using improper sample (not EDTA), spontaneous agglutination (cells heavily coated with IgM), non-specific agglutination (sticky cells)

Question 3

Function of check cells

Answer 3

to make sure the AHG was added after wash & make sure reagent is working

Question 4

Elution

Answer 4

procedure that removes antibody bound to the RBC, this antibody goes into the eluate, which can be used for serologic testing

Question 5

Adsorption

Answer 5

process of adding specific antigen so that antibody can attach to it & be removed from a serum, the antigen adsorbs the antibody

Question 6

Absorption

Answer 6

process of removal of antibody from a serum

Question 7

IAT

Answer 7

RBC antigen typing, antibody detection and ID, Weakened D test, crossmatch… detect In vitro sensitization of red cells

Question 8

DAT

Answer 8

Detects In vivo coating of patient cells (IgG antibodies, complement, or both)

Question 9

Purpose of pre-transfusion testing

Answer 9

Ensures ABO compatability between the donor and the recipient and should detect most clinical significant red cell alloantibodies that will react with donors red cells

Question 10

Major

Answer 10

pt serum/plasma

donor cells

Question 11

Minor

Answer 11

donor serum/plasma

pt cells

Question 12

RBC Frozen High Glycerol

Answer 12

10 yrs/ -65 degrees C

Question 13

Whole Blood

Answer 13

12 months/ 1-6 degrees C

Question 14

Whole Blood with Heparin

Answer 14

Mostly used for open heart surgeries, must be used within 24-48 hrs of collection

Question 15

Whole Blood CPD

Answer 15

21 days/ 1-6 degrees C

Question 16

RBCs CPD

Answer 16

21 days/ 1-6 degrees C

Question 17

RBCs open system

Answer 17

24 hrs/ 1-6 degrees C

Question 18

RBCs with Adenine saline additive

Answer 18

42 days/ 1-6 degrees C

Question 19

Platelets

Answer 19

5 days, 20-24 degrees C

Question 20

RBCs Deglycerolized

Answer 20

24 hrs/ 1-6 degrees C

Question 21

Cryoprecipitate

Answer 21

12 months/ -18 degrees C