Exam 3 - Calf & Assisted Techniques

Question 1

Death < 2 days old is due to what

Answer 1

non-infectious

Question 2

Death > 3 days is due to what

Answer 2

infectious

Question 3

concerns with neonatal calves

Answer 3

hypoxemia
hypercapnia/resp acidosis
high lactate/metabolic acidosis
hypothermia

Question 4

what is the number one cause of abnormal neonatal adaptation

Answer 4

dystocia

Question 5

how to provide assistance to abnormal calves

Answer 5

1. stimulate & enhance respiration
2. maintain body temp
3. increase blood volume and provide energy via colostrum

Question 6

what hormones do you give for superovulation in cattle

Answer 6

FSH or eCG

Question 7

method for embryo transfer

Answer 7

non-surgical, transcervical uterine lavage

Question 8

where to place the embryo in a transfer in a cow? horse?

Answer 8

Cattle – embryo placed on the same side as CL present (local prostaglandins)

Horse – embryo can be placed on either side (systemic prostaglandins)

Question 9

how can you determine embryo sex?

Answer 9

aspiration of blastomeres or biopsy of blastomeres
DNA probes can detect male cells (PCR)

Question 10

when do you transfer an embryo?

Answer 10

Transfer at late morula to blastocyst stage into a synchronized recipient

Question 11

how can you determine the difference between a X and Y bearing sperm

Answer 11

X bearing sperm have 3% more DNA (brighter) than Y bearing sperm

Question 12

true or false
EQ breed associations will NOT allow registrations of cloned horses

Answer 12

true

Question 13

where to collect sperm?

Answer 13

tail of epididymis and vas deferens