EXAM 2 P1 Flashcards
culture media
defined as medium vs. complex medium
defined medium
aka minimal medium
made with purified chemicals
glucose and amino acids
we know the concentration
complex medium
aka undefined medium
made with some nutrient source
yeast and soy beans
don’t know or care about the concentration
defined growth medium
works well with species with high biosynthetic capacity
what is biosynthetic capacity
making your own vitamins and nutrients
complex growth medium
needed for species with a lot of nutrient requirements
pathogens
selective media
prevent the growth of many things, while still allowing the growth of others
differential media
allow more things to grow, giving colonies a different appearance with dyes
liquid medium
rapid and constant mixing
simple recovery of cells for further studies
solid medium
isolation of individual colonies and pure cultures
can observe phenotypes and count the number of cells
bacterial cell division
done through binary fission
cells grow large and decide to divide, usually symmetric down the middle. binary fission can be observed with fluorescent microscopy
cytokinesis
process of splitting the cytoplasm in 2
involves septum formation
septum
structure built to come down in between 2 halves of the cell and pinch off the membrane
divisome
creates the septum
made of proteins beginning with Fts
Fts proteins
filamentation temperature sensitive
FtsZ
homolog of tubulin found in eukaryotic cells that form a filament around the cell’s center
FtsA and ZipA
linked to the cytoplasmic membrane and connect to FtsZ.
hydrolyzes GTP
FtsI
extends outside the membrane and is directly involved in synthesizing peptidoglycan
conditional mutant
alteration of a gene or protein that works under some conditions
Min proteins
help find the cell center
comes from minicell formation
Min E
goes to cell poles and has an affinity for the curved membrane.
when at one pole it drives Min C and D out and oscillates it before going to the next one
Min C and D
inhibit divisome formation by creating FtsZ
minicells
mutants that divide near one pole rather than the middle
do NOT have a chromosome
produces a functional metabolizing cell with NO DNA
