Exam 2: Cell Adaptation and Injury Flashcards
Why do animals become sick or diseased?
Sick cells result in sick animals
Cellular dysfucntion— organ dysfunction— animal dysfunction
What is the normal cell confined to a fairly narrow range of function and structure by?
Genetic programs of metabolism, differentiation, and specialization
Constraints of neighboring cells
Availability of metabolic substrates
What are the cellular responses to stress?
More severe physiologic stresses and some pathologic stimuli cause physiologic and morphologic cellular adaptations
Achieve new but altered steady states
Preserve the viability of the cell
Modulate function as cell responds to stimuli
What happens if the limits of adaptive response to stimulus are exceeded or if a cell is exposed to an injurious agent or stress?
Cellular injury results
What happens if the stimulus persists or is severe enough from the beginning?
The cell reaches “the point of no return” and suffers irreversible cell injury and cell death
Describe normal cells and adaptations
Cells have specialized functions and specialized structures based on their functions
All cells have certain “standard” organelles
—Synthesis of lipids, proteins, CHOs
—Energy production
—Transport of ions and other molecules
What is homeostasis?
Tight control of pH, electrolyte concentration, etc
What does departure from homeostasis lead to?
Cell damage
How do cells respond to homeostatic challenges?
By adpatation
What results if a new level of homeostasis cannot be achieved?
Cell death
What are examples of cellular adaptations?
Increase in muscle mass with exercise
Increase in cytochrome p450 mixed function oxidation expression in hepatocytes
How do cells adapt?
By either increasing or decreasing content of organelles
Atrophy, hypertrophy, hyperplasia, metaplasia
What is atrophy?
Reduction in mass of a tissue or organ
What is hypertrophy?
Increase in the size of cells, resulting in enlargement of organs
What is hyperplasia?
Increased number of cells in an organ or tissue
What is metaplasia?
Transformation or replacement of one adult cell type with another
What can atrophy be due to?
Either loss of cells or reduction in the size of cells within an organ
What are the adaptive responses to altered demands with cellular atrophy?
Decreased workload Decreased nutrition Loss of hormonal stimulation Decreased blood supply Loss of innervation
What occurs with cellular atrophy?
Reversible cellular change
Reduced functional capacity
Continue to control internal environment and produce sufficient energy for metabolic state
What may prolonged cellular atrophy lead to?
Death of some of the cells
Loss of muscle cells with prolonged denervation
Atrophy at the organ level may become irreversible at this point (muscle) or may be reversible by hyperplasia (liver)
Describe hypertrophy at the organ level
Hypertrophy increases organ size without cellular proliferation
Is cell enlargement in hypertrophy that same as cell swelling?
No, it is different
What are changes in hypertrophic cells?
Increased protein content Increased organelle number ---Myofibrils (muscle) ---Mitochondria ---ER
Is the anabolic process or catabolic process greater in hypertrophy?
Anabolic, breaking down quicker than we are building up
Why may hypertrophy not be advantageous to an animal?
Point of diminishing returns
Heart
-Hypertrophy does not change underlying problem and cannot provide adequate energy/contraction
-Conformational changes associated with hypertrophy decrease ejection volume
-May eventually end up with organ failure
What is hypertrophy in the liver?
Induction
Describe induction
Effect may be helpful or not helpful:
Tolerance to certain drugs/toxins
More rapid removal of certain drugs/toxins
May increase susceptibility to some toxins
–Metabolites may be more toxic than precursors
What is cell injury?
Any change that results in loss of the ability to maintain the normal or adapted homeostatic state
What happens when cells become unable to balance the processes that regulate internal environment?
We see morphologic changes that we recognize as cell injury or degeneration
What does the extent of cell injury depend on?
Severity of stimulus
Type of cell involved
Metabolic state at the time of injury
What is reversible cell injury?
Degeneration
What are hallmarks of cell degeneration?
Cell swelling Fatty change Lipofuscin and ceroid Hyaline changes Amyloid Mucinous change Calcification Gout Cholesterol crystals Inclusions
What is an early, almost universal sign of cell injury?
Cell swelling
Describe cell swelling
Cells enlarged
Compress adjacent structures (loss of sinusoids in liver)
Altered staining characteristics (pale, cloudy appearance, cytoplasmic vacuoles caused by distended organelles, lipid droplets)
Loss of energy control/production
Not incompatible with life of the cell and is often mild and rapidly reversible
Also occurs in lethal injury
What does cell swelling result from?
Loss of control of ions/water with net uptake of water
What is the gross appearance of cell swelling?
Organ is often pale
Enlarged, swollen (rounded margins, heavy, wet)
Bulges on cut surface
What is the effect of cell swelling in the brain?
Severe effects due to pressure necrosis
What is the effect of cell swelling in the liver?
Decreased blood flow, decreased function
What is the effect of cell swelling in the pharynx?
Airway obstruction
What is fatty change?
Accumulation of neutral fats (TG) in a cell
Nothing to do with adipose tissue
What is fatty change common in?
Injured cells, especially cells that metabolize lots of lipids
What do sick cells tend to accumulate?
Triglycerides
What is the gross appearance of fatty change?
Yellow discoloration (kidney/liver) Enlarged (liver)
What is the pathogenesis of fatty change?
Overload (increased mobilization of fats; diabetes mellitus)
Injury to cells (toxins, anoxia)
Deficiencies (methionine, choline)
When is lipidosis normal?
In young animals (milk diet)
Following fatty meals
What is seen grossly with glycogen accumulation?
Swollen organ
Rounded margins
Increased pallor
What is seen microscopically with glycogen accumulation?
Enlarged cells
Increased pallor
No nuclear displacement
What is the pathogenesis of glycogen accumulation?
Prolonged, severe hyperglycemia (diabetes mellitus) Increased corticosteroids (Cushing's, iatragenic) Enzyme deficiencies (glycogen storage diseases, defects in a step of glycogen breakdown)
What is lipofuscin?
Pigment that collects in cells