Exam 2 Applied and Environmental Microbiology Flashcards
Use of microorganisms in food production to prevent spoilage and illness
Food microbiology
Application of microbes to industrial manufacturing processes
Industrial microbiology
Three functions of microbial metabolism
Act as preservative
Destroy pathogenic microbes and toxins
Add nutritional value
Any desirable change that occurs to a food or beverage as a result of microbial growth
Fermentation
Composed of known microorganisms that consistently perform specific fermentations
Starter cultures
Adverse changes to a food due to the action of microorganisms
Spoilage
Bread
Beer
Sake
Whiskey/Vodka
Grain-based Fermentations
Sauerkraut Hot pepper sauce Olives Kimchi Pickles
Vegetable-based fermentations
Miso
Soy Sauce
Tempeh
Bean-based fermentations
Chocolate
Coffee
Vanilla
Bean-based fermentations (different bean)
Wine
Some liquors
Vinegar
Kombucha
Fruit-based fermentations
Mead
Honey-based fermentations
Buttermilk
Yogurt
Cheese
Kefir
Dairy-based fermentations
Official State Microbe of Wisconsin
Lactococcus lactis
Salami
Pepperoni
Prosciutto
Meat-based fermentations
Nutrient rich, moist, and unprotected foods with MINIMAL storage time
Perishable
Can store sealed for months without spoiling
Semi-perishable
Dry or canned foods that can be stored indefinitely
Non-perishable
Sugar and salt remove water from food. They are known as this
Preservatives
This temperature prevent food spoilage
High temps
This temperature is desirable for food storage
Low temperature
Consumption of living microorganisms
Food infections
Consumption of microbial toxins rather than the microbe
Food intoxications
38% (the highest amount) of foodborne illnesses come from this
Food service industry
25% of foodborne illnesses come from this
Home preparation
Most common cause of diarrhea of all foodborne agents
Campylobacter jejuni
These two forms of bacteria grow at fridge temps
Yersinia enterocolitica
Listeria monocytogenes
Second most common cause of foodborne illnesses
Salmonella
Third most common cause of foodborne illnesses
Shigella
Number one cause of foodborne illnesses
Norovirus
Where is industrial microbiology used?
Industrial fermentation Industrial products Water and wastewater treatment Disposal and cleanup of biological wastes Treatment of mine drainage
Physical water pollution
Presence of particulate matter
Chemical water pollution
Presence of inorganic or organic compounds
Biological water pollution
Too many or non-native microorganisms (polluted waters)
Vibrio cholerae
Cholera
Campylobacter jejuni
Acute gastroenteritis
Salmonella spp.
Salmonellosis, typhoid fever
Shigella spp.
Shigellosis (bacterial dysentery)
Escherichia coli
Acute gastroenteritis
Hepatitis A virus
Infectious hepatitis
Norovirus
Acute gastroenteritis
Poliovirus
Poliomyelitis
Giardia intestinalis
Giardiasis
Schistosoma spp.
Schistosomiasis
Entamoeba histolytica
Amebic dysentery
Water that is considered safe to drink
Potable water
Presence of this in water indicates fecal contamination
Coliforms
What are the 4 stages for the treatment of drinking water
Coagulation and flocculation
Sedimentation
Flitration
Disinfection
In the coagulation and flocculation stage, this chemical is added to help the flocs settle to the bottom
Alum
In the sedimentation stage, what is removed from the water?
Large and particulate materials
In the filtration stage, what are three methods of removal?
Sand filtration
Activated charcoal
Membrane filtration
In the disinfection stage, inactivation of remaining microbes can occur by what 3 methods?
Chlorine
Ozone
UV light
What are the 4 stages for traditional wastewater treatment?
Primary treatment: sedimentation
Secondary treatment
Chemical treatment
Sludge treatment
What percentage of BODs are removed during primary treatment of wastewater?
25-35%
What percentage o BODs are removed during secondary treatment of wastewater?
75-95%
Describe the chemical treatment of wastewater.
Chlorination and/or filtration prior to release
Describe the sludge treatment of wastewater
Anaerobic digestion of sludge and the methane is burned off or trapped for fuel
Uses organisms to clean up toxic, hazardous, or recalcitrant compounds by degrading them to harmless compounds
Bioremediation
Microbes encouraged to degrade toxic substances in soil or water or addition of nutrients to stimulate microbial growth
Natural bioremediation
Genetically modified microbes specifically degrade certain pollutants
Artificial bioremediation
Uses microbes or their toxins to terrorize human populations
Bioterrorism
Uses microbes to terrorize human populations by destroying their food supply
Agroterrorism
What is the criteria for assessing biological threats to humans?
Public health impact
Delivery potential (how easily it’s introduced)
Public perception
Public health preparedness
Variola major (orthopoxvirus)
Smallpox
Bacillus anthracis (bacterium)
Anthrax, source is soil
Yersinia pestis (bacterium)
Plague, source is small rodents
Clostridium botulinum toxin (bacterial)
Botulism, source is soil
Francisella tularensis (bacterium)
Tularemia, source is wild animals
Filoviruses and arenaviruses
Viral hemorrhagic fevers, source is unknown
Handling pathogens that do not cause disease in healthy humans
Biosafety level 1
Handling of moderately hazardous agents
BSL-2
Handling of microbes in safety cabinets since they may cause serious or potentially lethal disease after inhalation
BSL-3
Handling of microbes that cause severe or fatal disease
BSL-4
What are two roles of recombinant genetic technology in bioterrorism
Could use to create new or modify biological threats
Could be used to thwart bioterrorism