Exam 1 Material Flashcards
Microbiology
Study of entities too small to be seen with the unaided human eye
This person developed a taxonomic system for naming plants and animals and grouping similar organisms together
Carolus Linnaeus
Genus species
Binomial nomenclature
What are the three domains?
Bacteria
Archaea
Eukarya
What are the four subdivisions of the eukarya domain?
Fungi
Protista
Algae
Animalia
What is the difference between bacteria and archaea?
Archaea do NOT cause disease
What did Leeuwenhoek term what he saw under magnification?
Animalcules
On the ancestral cell line, which domains branch off the same root?
Archaea and Eukarya
Another term for spontaneous generation
Abiogenesis
What is spontaneous generation?
Living organisms can arise from nonliving matter
Who proposed spontaneous generation?
Aristotle
Who challenged spontaneous generation with meat in a flask?
Francesco Redi
Who was a supporter of spontaneous generation?
John Needham
Who challenged spontaneous generation by boiling broth in a flask?
Lazarro Spellanzani
Who is the father of microbiology?
Leewenhoek
Who is the father of MODERN microbiology?
Pasteur
Who ended spontaneous generation by preforming experiments in a swan neck flask?
Pasteur
What is the sugar fungus that makes beer?
Saccaromyces cerevisiae
Who developed the germ theory of disease?
Pasteur 1857
Study of causation of disease
Etiology
Who discovered the cause of anthrax and tuberculosis?
Koch
What is the bacteria that causes anthrax?
Bacillus anthracis
What is the bacteria that causes TB?
Mycobacterium tuberculosis
What was a technique of Koch that we use today?
Method of isolation - agar
What are Koch’s postulates used for?
Steps that must be taken to prove the cause of any infectious disease
What are Koch’s four postulates?
Suspected agent must be found in every case of disease and absent in healthy hosts, agent must be isolated and grown outside host, when agent is introduced into a healthy susceptible host they must get the disease, and same agent must be found in the diseased experimental host
Hand washing
Semmelweis
Weis - Wash
Antiseptic technique
Lister
think Listerine
Cleanliness techniques in nursing
Nightingale
prof Nightingale dressed as a nurse
Infection control/field of epidemiology
Snow
Smallpox vaccine/field of immunology
Jenner
Magic Bullets/field of chemotherapy
Ehrlich
Cadaver particles caused what disease in women in labor?
Puerperal fever
Study of the occurrence, distribution, and spread of disease in humans
Epidemiology
John Snow was the foundation for what two branches of micro?
Infection control
Epidemiology
Chemicals are studied for their potential to destroy pathogenic organisms
Chemotherapy
A condition acquired from eating infected beef
variant Creutzfeldt-Jakob disease
In vCJD, the brain is full of sponge-like holes known as what?
Spongiform encephalopathy
What are the four processes of life?
Growth
Reproduction
Responsiveness
Metabolism
What is the root of something that responds to the environment?
-taxis
What are the two main features of prokaryotes?
Lack nucleus
Circular DNA
What are the two main features of eukaryotes?
Have nucleus
Linear DNA
What are two types of glycocalyces on bacterial cells?
Capsule and slime layer
Firmly attached to cell surface and may prevent bacteria from being recognized by host
Capsule
Loosely attached to cell surface and provides a sticky surface
Slime layer
Responsible for movement of some bacteria
Flagella
When a bacteria runs, what is the movement like?
Straight line
When a bacteria tumbles, what is the movement like?
Spins
What are bacterial cell walls composed of?
Peptidoglycan
What are the two basic types of bacterial cell walls?
Gram + and Gram -
Gram + have a thick layer of peptidoglycan and appear what color?
Purple
What are the unique polyalcohols in Gram + cells called which provide rigidity?
Teichoic acids
Gram - bacteria have a thin layer of peptidoglycan and appear what color?
Red
Bacterial cytoplasmic membranes maintain what two gradients?
Concentration and electrical gradient
List 3 passive processes
Diffusion
Facilitated diffusion
Osmosis
List 2 active processes
Active transport
Group translocation
Substance is chemically modified during transport and no longer a viable candidate to go back out of the membrane
Group translocation
Osmosis goes from what concentration to what concentration?
From low to high concentration
There is the same concentration inside and outside the cell
Isotonic solution
There is more concentration inside the cell, so water rushes in resulting in rupture of the cell
Hypotonic solution
There is more concentration outside the cell, resulting in crenation or dehydration
Hypertonic solution
Unique structures produced by some bacteria that are a defensive strategy against unfavorable conditions
Endospores
What is the size prokaryotic ribosomes?
70S
What are the two subunits of prokaryotic ribosomes?
30S and 50S
Do archaea cell walls contain peptidoglycan?
Do NOT have peptidoglycan
Animals and most protozoan cells lack this
Cell walls
Cells using endocytosis will form this to bring something into the cell
Pseudopodia
Solid imported into cell
Phagocytosis
Liquid imported into cell
Pinocytosis
Eukaryotic flagella do not rotate but do this instead
Undulate rhythmically
Do prokaryotic cells have cilia?
No prokaryotic cells have cilia
What is the size of eukaryotic ribosomes?
80S
What is the size of 2 subunits of eukaryotic ribosomes?
40S and 60S
In the mitochondria and chloroplasts of eukaryotes, what is the size of their ribosomes?
70S
Plants, algae, most fungi, and prokaryotes lack this, a structure that plays a role in mitosis
Centrioles
This is the control center of the cell
Nucleus
This functions as the transport system in the eukaryotic cell
Endoplasmic reticulum
This organelle receives and packages molecules but is not present in all eukaryotic cells
Golgi body
This is the second most important organelle in the cell that is known as the powerhouse of the cell
Mitochondria
Prokaryotes lack this organelle which is a light-harvesting structure
Chloroplasts
The shortest distance between two points on a specimen that can still be distinguished by the observer as separate entities
Resolution
Differences in intensity between two objects, or between an object and background
Contrast
Name 4 simple stains
Crystal violet
Safranin
Methylene blue
Malachite green
Name 3 differential stains
Gram stain
Acid-fast stain
Endospore stain
Name 2 special stains
Negative stain
Flagellar stain
List the 4 steps of Gram staining
Flood with crystal violet
Iodine = mordant
Ethanol and acetone solution
Safranin = counter stain
This type of bacteria does not have peptidoglycan cell walls but a waxy mycolic acid content instead
Mycobacteria
Due to the waxy walls of mycobateria, what staining procedure is used?
Acid-Fast stain
What colors are produced in acid-fast stains?
Red- acid fast
Blue- negative for acid fast
What colors are produced in endospore staining?
Green- endospore
Red- vegetative cell
Series of paired statements where only one of two “either/or” choices applies to any particular organism
Dichotomous keys