Exam 2 Flashcards
What was Leeuwenhoeks contribution to science
first observation of living microorganisms
What was hooks contribution to science
First sell observation using cork. The cell was dead
Compare the magnification potential of light and electronic microscopes
Electron has a higher magnification so you can see more
Which type of cell is bigger
Eukaryotic
Which type of cell is more complex
Eukaryotic
Which type of cell contains a nucleus
EUK a RYOT I C
Which type of cell contains organelles
Eukaryotic
Describe the DNA chromosome in prokaryotic cells
Single and circular
Describe the DNA chromosomes in eukaryotic cells
Multiple and linear
What was Woeses contribution to science?
He used a specific DNA sequence as a basis for comparison for all types of cells. Used rRNA
What are the 3 domains proposed by Woese?
Bacteria archaea and eukarya
What did Rosie used to create the new phylogenic tree
A specific DNA sequence
Is the importance of the plasma membrane
Permeability membrane
What molecules can be components of the plasma membrane
Composed of phospholipids and proteins and sometimes Sterols
What are the properties of phospholipids, sterols, and proteins that allow them to interact together?
Hydrophilic and hydrophobic
What is the function of ribosomes?
To make proteins found in the cytoplasm from amino acids
What is the subunit composition of ribosomes?
Large and small subunit
What is the composition of the large subunit?
Proteins and rnas
What is the composition of the small subunit?
Proteins and RNAs
What is the bacterial ribosome called?
70S
What is the eukaryotic ro some called?
80S
In which type of cell would the following structures be found: Nucleoid Nucleus Organelles Cytoplasm Cytosol Fimbriae Flagella Cell wall Capsule Cilia
P E E E E P B B P E
What is the funtion of flagella?
Motility
What is the function of fimbriae
Attachment
What is the function of cilia
Motility
What is the function of the cell wall?
Protection
What is function of capsules?
Protection
How is the nucleoid different than the nucleus?
Nucleoid a non-membrane and us organization of DNA; nucleus is a membrane-enclosed structure with specialized functions
What do Histone proteins do?
They compact and organize all DNA inside the nucleus
How is chromatin diff than chromosomes?
Chromosomes plus Histone proteins make up chromatin
Which organelle is specific to animal cells?
Lysosomes
Which organelle is specific to plant cells?
Chloroplasts and vacuoles
Which organelles are found in both animal and plant cells?
Nucleus, rough ER, Golgi apparatus, smooth ER, and mitochondria
Which organelles deal mostly with proteins?
Rough ER and Golgi
Which organelles deal mostly with carbs?
Mitochondria and chloroplasts
Which organelle deals mostly with lipids
Smooth ER
Which organelles are double-membrane organelles?
Nucleus, mitochondria, and chloroplasts
Which organelle is physically attached to the nucleus?
Rough ER
What is the small structure inside the nucleus called?
Nucleolus
What is the function of ribosomes
To make proteins
What is the function of the nucleolus
Assembles ribosomes
What is the composition of ribosomes?
Two subunits composed of proteins and RNAs
What are the two important cell processes involved DNA that occur inside that nucleus?
Replication and transcription
Where inside eukaryotic cells does DNA replication occur?
Nucleus
Where inside eukaryotic cells does does transcription occur?
Nucleus
What molecules and/or macromolecules can enter and leave the nucleus thru the nuclear pores?
RNA and certain proteins
What are the two main functions for the smooth ER mentioned in lecture?
Synthesis of lips and detoxification of poisons
What is the main function of he rough ER?
Produce secretory proteins ( protein factory)
What is the main function of the Golgi?
Modification of proteins, involved in protein trafficking (it’s like Amazon)
What is the organization of the Golgi relative to the rough ER and plasma membrane?
It’s the packaging and processing part for proteins
Which side of the Golgi is the cis and which is trans?
Cis is on side of rough ER and trans is plasma membrane
What is a vehicle
Transporting unit
How is a vesicles diff than an organelle
Vehicle doesn’t really do much only involved in movement while the organelles are very important aspects to the cell
Describe endocytosis
Involved in the secretion of molecules into the surrounding environment
Describe exocytosis
Involved in the secretion of molecules from the environment
How are vesicles involved in endo and exocytosis
Vesicles are involved in the movement in or out
What is the main function of lysosomes
Digestion and Degradative processes ( breaking down)
What are 3 things that aid in the lysosomes in digestion
Hydrolysis enzyme, reactive molecule, and acidic interior
What are the two activities of the lysosomes?
Autopsy and phagocytosis
Describe autophagy
Digestion and recycling of damaged organelles
Describe phagocytosis
Digestion of engulfed cells or particles
What is the main function of the vacule in plant cells
Storage unit in plants
What was the contribution to science by Lynn margulis?
Endo symbiotic theory
What does the endo symbiotic theory state?
Chloroplasts and mitochondria have a very similar structures and functions to bacteria margulis thought since they were so similar they were once bacteria but a cell engolf them and took over
What pieces of evidence support the endosymbiotic theory?
The structure of the mitochondria and chloroplasts such as the double membrane organelles involved in aerobic respiration they contain their own ribosomes and chromosomal DNA and capable of independent division
What are the steps in protein trafficking?
- Protein produced by ribosomes on rough ER
- Protein leaves tough ER in vesicle
- Enter the cis side of Golgi
- Travel thru Golgi; leave Golgi in a vesicle
- Travel to final destination
Which organelles are involved in protein trafficking before proteins reach their final destination?
Rough ER, Golgi, lysosomes
Where do proteins originate in this process?
Rough ER
Where do proteins travel to after leaving the rought ER?
Cis side of Golgi
In what structure do proteins travel from the rough ER to the Golgi?
Vesicle
Which side of the Golgi do proteins enter?
Cis
Which side of the Golgi do proteins leave from?
Trans
Where do proteins go after leaving the Golgi?
Lysosomes, insertion into plasma membrane, secretion outside into environment
In what structure do proteins travel after leaving the Golgi?
Vesicle
What are the 3 cytoskeleton protein filaments?
Microtubules, intermediate filaments, micro filaments
Which crypto skeletal protein filament is the biggest?
Microtubles
Which cytoskeleton protein filament is the smallest?
Microfilaments
What activity do all three cytoskeletal protein filaments have?
Support and structure
What protein are microtrubles composed of?
A and b tubulin
What are the three movement activities that use microtrubles
Vesicle movement, flagella/ cilia movement, and chromosome movement
Which motor protein is used for vesicle movement?
Kinesin
Which motor protein is used for flagella and cilia movement
Dynein
Which motor protein is used for chromosomes movement?
Kinetochores
What protein are microfilm ends composed of?
Actin
What are the 3 movement activities that use micro filaments?
Cell movement, animal cell division, and cytoplasmic streaming ( organelle movement)
What motor protein is used for microfilament associated movements?
Myosin
What does cytoplasmic streaming refer to?
Organelle movement
What is a pseudopodia and what does it do?
It’s the ameboid movement
What is the function of intermediate filaments
Cell shape
What are the functions of the membrane?
Cell boundary, permeability barrier (transport control and electrical/chemical gradient), site for protein attachment/insertion
What is the major component of the membrane
Phospholipids
Hydrophilic
Interacts with water, likes water
Hydrophobic
“Scared” of water, doesn’t like water
What is the difference between a saturated fatty acid and and unsaturated fatty acid
Unsaturated is bent saturated is straight
Describe the characteristics of a membrane that only contain saturated fatty acid
If there are too many saturated acids since it will be hard and not permeable at all
Describe a membrane that only contains unsaturated fatty acid
It will be too liquidy
What role does cholesterol play in terms of the membrane
Affect /change membrane permeability
What are the two general types of membrane proteins
Peripheral and integral
Where are peripheral membrane proteins located
On the outside of the membrane
What are the potential activities of peripheral membrane proteins
Enzymatic, hydrolytic, communication, signaling, and recognition
Where are the integral membrane proteins located
On the inside of the membrane in between phospholipids
What are the potential activities of integral membrane proteins
Transportation
Define passive movement
No energy is required, molecules move from an area of high concentration to an area of low concentration
Define active movement
Energy is required molecules move from an area of low concentration to an area of high concentration
Compare and contrast passive and active movement
Passive results and dilution an active results and concentration
Which type of movement requires energy
Active
Which type of movement tries to dilute
Passive
Which type of movement tries to concentrate
Active
What is a solution
Solute plus solvent
What is a solute
solid
What is a solvent
“Liquid” normally water
What are the three types of passive movement?
Simple diffusion, facilitated diffusion, and osmosis
Diffusion
Movement of a substance from a high concentration to a low concentration
Define simple diffusion
Solutes move through the membrane unassisted
Define facilitated diffusion
Solutes move through a transport protein in the membrane
Compare and contrast simple and facilitated diffusion
Simple is unassisted
Which type of diffusion requires transport proteins
Facilitated
When our at what point does diffusion stop
When it reaches an equilibrium
What kinds of molecules are freely diffusible
Small nonpolar and polar compounds
What kinds of molecules are not able to freely cross the membrane
Large or uncharged molecules
Define osmosis
Water moves through the membrane unassisted high to low
Define hypotonic solution
Solute concentration outside the cell is lower than inside of the cell
Define isotonic solution
solute concentration outside the cell is equal to inside the cell
Define hypertonic solution
Solute centration outside the cell is higher than inside the cell
Which direction will water move when A cell is in a hypotonic solution
Into
What happens to the cell in a hypotonic solution
Leads to osmotic lysis
Which direction water move when A cell is in a hypertonic solution
Out
What happens to the cell in a hypertonic solution
Plasmolysis
Which direction will water move when A cell is an iso tonic solution
It won’t
What happens to the cell in an iso tonic solution
Equilibrium
What is osmotic lysis
The influx of water into a cell, will cause the cell to swell and burst
In which solution is osmotic lysis possible
Hypotonic
Which cell structure protects against osmotic lysis
Cell wall
What is plasmolysis
Deflux of water out of the cell, cytoplasm shrinks and pulls away from the membrane
In which solution is plasmolysis possible
Hypertonic
What is the difference between a channel protein and a carrier protein
Channel proteins allow certain molecules to pass through, carrier bind and carry Molecules through
What is the difference between an open channel and A gated channel
Open let’s anything through gated is selected
Rank the following from a specific to least
Carrier protein
Gated
Open
What is the overall, balanced chemical reaction for aerobic respiration or cellular respiration?
C6H12O6 + 6⃣O2 –> 6⃣H2O + energy
What organelle is needed for aerobic respiration
Mitochondria
Does aerobic respiration produce or consume energy and how much
Produce 30 ATP
What is the overall balanced chemical equation for photosynthesis
6⃣CO2*6⃣H2O + energy –> C6H12O6 + 6⃣O2
What organelle is needed for photosynthesis?
Chloroplasts
Does photosynthesis produce or consume energy and how much?
consume 18 ATP
Degradative
One thing going to many things
Breaking down
Big thing going to small things
Exergonic
Nonpolar to polar
Catabolic
Hi energy things going to low energy things
Spontaneous
Ordered things going to disordered
Oxidation
Loss of electrons, gain of oxygen
Reduction
Gain of electrons
How can hydrogen and oxygen be used to determine reduction
Gang of hydrogen loss of oxygen
What does a redox couple refer to
Oxidation and reduction
What does a redox couple represent?
Oil rig: oxidation (loss of e-, loss of H, gain of O) reduction (gain of e-, gain of H, loss of O)
What are the three steps of aerobic respiration?
- Glycosis
- TCA cycle
- Electron transport chain
What is the significance of the consumption of 2 ATP during glycolysis?
Goes towards the 30 ATP
What happens to NAD+ during glycolysis?
They reduction it changes to NADH
What is the pyruvate in the process of glycolysis
The product
What is the purpose of electron carriers
They’re cofactors
What do electron carriers doc
They accept and donate electrons
How are electron carries related to coenzymes?
NAD+ and FAD are coenzymes