Exam #1: Physiology of the ANS II Flashcards
What muscarinic receptors are present in the heart? What nervous system are they associated with?
- M2
- PNS
What type of G-protein is associated with the M2 receptor in the heart?
Gi/o= inhibitory G-protein
Decrease cAMP & PKA
What do M2 receptors inhibit in the heart?
- SA node= negative chronotrope
- AV node= decreased conduction velocity
- Atrial muscle= decreased atrial contracion
- Ventricular muscle= decreased ventricular contracion
What type of muscarinic receptor is present in the lungs?
M3
What is the effect of M3 activation in the lungs?
- Contraction of the bonchi & bronchioles
- Secretion from submucosal glands
*DO NOT use drugs in asthma patients that have PNS activity
What type of muscarinic receptor is present in the stomach? What is the effect of stimulation of these receptors?
M3, motility & cramps
What type of muscarinic receptor is present in the glands? What is the effect of stimulation of these receptors?
M1, secretion
What type of muscarinic receptor is present in the intestine? What is the effect of stimulation of these receptors?
M3, contraction–diarrhea & involuntary defecation
What is the general clinical picture of ACE inhibitor intoxication?
Voiding, urinary incontinence
V. wet
What is the difference between a coupled cell & an indirectly coupled cell?
- Directly coupled= directly activated by a neurotransmitter released from a varicosity
- Coupled= junction potentials spread from directly innervated cells
- Indirectly coupled= a sufficient area of a muscle bundle is depolarized to propagate the action potential to the cell
What does the innervation of the visceral smooth muscle differ from the vascular smooth muscle?
Visceral smooth muscle receives input from one source.
Vascular smooth muscle receives input from 2x sources:
1) Perivascular nerves
2) Endothelial input
What effects are mediated by perivascular nerves?
- A number of neurotransmitters released from varicosities in the tunica adventita act on receptors in the media, leading to vasoconstriction.
What effects are mediated by endothelial input?
- In response to shear stress or hypoxia, endothelial cells release factors that mediate vasodilation (Endothelium-Derived Relaxing Factors)
What is the effect of damage to the endothelial cell layer?
- When the Endothelium is damaged, activation of M3R on vascular smooth muscle causes vasocontriction (unopposed by EDRFs)
Outline the pathway that underscores the effects of M3R activation in the blood vessels.
1) ACh binds to M3
2) Increase intracellular Ca++
3) Increase in Nitric Oxide Synthase
4) Increased NO production via the endothelial cell
5) Relaxation
Why is vasoconstriction the outcome if the endothelium has been damaged?
Ca++ increase leads to contraction of the smooth muscle, but there is not the opposing NO, ultimately resulting in vasoconstriction.
Describe the mechanism of M1 & M3 receptor activation.
1) Receptor activation
2) Gq (activation)
3) PLC
4) PIP2–>IP3 & DAG
5) Increase Ca++ & PKC
Describe the mechanism of M2 receptor activation.
1) Receptor activation
2) Gi
3) Adenylul Cyclase inhibited
4) Decrease cAMP
5) Decrease PKA
Name the three locations of nicotinic ACh receptors.
1) PNS ganglia
2) Targets of the somatic nervous system, skeletal muscle
3) Adrenal medulla
What type of nicotinic receptor is present in the adrenal medulla? What is the response to ACh binding?
- Nn
- Secretion of Epi & NE
What type of nicotinic receptor is present in the autonomic ganglia? What is the response to ACh binding?
- Nn
- Stimulation
*Note that all autonomic ganglia have nicotinic AChR
What type of nicotinic receptor is present in the neuromuscular junction? What is the response to ACh binding?
- Nm
- Stimulation i.e. twitch & hyperactivity of skeletal muscle
Remember skeletal MUSCLE, N-M
How are catecholamines synthesized?
1) Tyrosine is converted to DOPA via Tyrosine Hydroxylase*
2) DOPA is converted to Dopamine
3) Dopamine is converted to Norepinephrine
Note that this is the rate-limiting step
What drug blocks tyrosine hydroxylase? What step of the catecholamine synthesis process does this drug block?
- Metyrosine
- Conversion of Tyrosine to DOPA
What part of catecholamine synthesis only occurs in the adrenal medulla?
Norepinephrine is converted to Epinephrine