Escherichia coli

Question 1

Specifics

Answer 1

Lactose positive
oxidase negative
motile rods
O antigen 26, K capsule 60 (B6), F fimbrae (41) and H flagella (11)

Question 2

E. coli in pigs

Answer 2

ETEC
VTEC
EPEC
UTI
MMA
Septicemia

Question 3

ETEC Virulence factors neonatal

Answer 3

F5, F6, F41
non hemolytic
*everything passed neonatal is hemolytic

Question 4

ETEC Virulence <4 weeks

Answer 4

F4, LT, EAST1

Question 5

ETEC Virulence factors Weaning diarrhea

Answer 5

F4, F18, LT, EAST1

Question 6

F4 Adhesion factor

Answer 6

pigs only, on plasmids,

Question 7

F5 Adhesion factor

Answer 7

pigs and bovine, only receptor for very young animals (neonatal), T dependent expression

Question 8

F6 Adhesion factor

Answer 8

extra chromosonal, neonatal

Question 9

F41 Adhesion factor

Answer 9

pigs and bovine, chromosomal

Question 10

F18 Adhesion factor

Answer 10

Edema disease

Question 11

ETEC Heat labile toxin

Answer 11

high molecular weight, strong Ag and used to make vaccines

Question 12

ETEC Heat stable toxin

Answer 12

low molecular weight, little antigenic

Question 13

ETEC Clinical signs

Answer 13

watery diarrhea, very smell feces, dehydration

Question 14

ETEC pathogenesis

Answer 14

ascend from lower bowel or oral uptake and then colonizes small intestine

Question 15

ETEC epidemiology

Answer 15

infection get from intestine of healthy sows
equilibrium between maternal immunity and infection pressure
low hygiene

Question 16

ETEC Tx and Prev

Answer 16

hydration
antimicrobials
vaccinate sows

Question 17

VTEC in pigs age affect

Answer 17

<3 weeks after weaning

Question 18

VTEC Clinical signs pig

Answer 18

Edema disease
swollen eye lids
hoarse voice
diarrhea with mixed and ETEC and VTEC

Question 19

VTEC virulence factor in pig

Answer 19

F18

Exotoxin- VT2e and STX2e media necrosis

Question 20

VTEC Prev and treatment in pigs

Answer 20

Affected pigs: fast to eliminate and then antimicrobial
Non affected pigs: antimicrobials and increase in 2-3 days
Prevent add zinc oxide

Question 21

EPEC (enteropathogenic) in pigs

Answer 21

rare
weaning pigs - diarrhea
virulence factor T3SS (needle and injection)
Diagnostics- Isolation and PCR (Eae- intimin gene)

Question 22

UTI in pigs

Answer 22

CS: anorexia, vaginal exudate

Tx/Prev: culling due to fertility problems, antibitotics for 3 weeks

Question 23

MMA in pigs

Answer 23

48 hours after farrowing
post partum dysgalactiae syndrome- no milk production, colostrum and septicemia
Edx: hereditary, hormonal, feed, infectious agents

Question 24

Septicemia in pigs

Answer 24

secondary to enteric infection

rarely primary

Question 25

ETEC in cows

Answer 25

age affected less than 3 days Dx: ELISA, culture and virulence factors (agglutination/PCR) Virulence factors: F5, F41, CS31A, STa, Stb Therapy: separate sick animals, hydration, antibiotics extremely high resistance, vaccinate dams, colostrum

Question 26

EPEC (enteropathogenic) EHEC (enterohemorrhagic)- Zoonotic in cow

Answer 26

``` Age 1-8 weeks CSS: mucoid diarrhea with/without blood mainly large intestine Therapy: antibiotics Virulence factors: EPEC- initimin eae, EHEC intimin eae + VT1 and VT2 ```

Question 27

Mastitis in cow

Answer 27

CS: udder swelling, redness, lesions prev/tx: hygiene and systemic Antibiotics no known virulence factors

Question 28

Septicemia in cow

Answer 28

CS: hyperacute- acute mortality and shock, acute- general symptoms, fast mortality, slower- organ localization: polyarthiritis, pneumonia, pleuritis, meningo- encephalitis Pathogenesis: PO--> lymph tissue--> septicemia / umbilical cord-->septicemia

Question 29

E. coli in dogs and cats (ETEC, EPEC, VTEC, EIEC)

Answer 29

Frequent with other pathogens | Virulence factor CNF1- Ecoli in puppies

Question 30

Cysitis/ pyometra

Answer 30

Cystitis inflammation in bladder, pyometra uterine infection cats seldom get cystitis, high osmolarity of urine Virulence factors: F12, F13 (associated with human UTI) Specific (o2,o4,o6,o83)

Question 31

E. coli in birds Clinical signs and Pathogensis

Answer 31

most important bacterial disease in broilers and layers Neonatal colibacillosus --> contamination of egg, neonatal or manipulation of chick that leads to yolk rest infection called omphalitis--> sepsis/death/retardation Respiratory colibacillosus--> excretion leads to increase of infection pressure--> inhaled--> cause infection or can damage resp epithelium Peritonitis--> decrease egg production

Question 32

Ecoli in birds- prevention

Answer 32

hygiene, decrease stress, control temp, vaccines, difficult to treat

Question 33

Ecoli is broilers

Answer 33

Serotype O78, O2 CS: scabby hips/necrotic dermatitis in broilers Use feathers for diagnosis, usually at slaughter Prev: low density of animals

Question 34

E coli in rabbits | RPEC/EPEC

Answer 34

virulence factor intimin eae Dx- isolate and identify serotype Tx- antibiotics