Brucella Flashcards
Specifics
non motile, coccobacilli, oblisgate symbiont
faculative intracellular
B. abortus
REPORTABLE
spread by females acute- general symptoms chronic intermittent fever (3-4wks) path target organ- uterus, abortion, premature calf is carrier subclinical mastitis dx milk samples for ag or ab
B. abortus in humans
acute bacteremic phase
chronic symptoms (years)- fever intermiottent, headache
muscle pain, enlarged lymph nodes, splenomegaly, osteomyelitis
virulence factors
no flagella or capsule thick peptidoglycan erythritol targets repro/mammary smooth cell wall rough cell wall- less virulent lacking O polysaccharide
pathogensis
PO/wounds–> lymoph node–> target organ
with no wsymptoms spread is intracellular
target organs- repro and joints
immunity
Abs come late
cellular immunity early
carriers- when cellimmunity is not capable of killing intracellular bacteria and immunity goes down
Dx
SAW SAT slow agglutination test
rose bengal test
CFT ELISA IFN y
SAW +–> double ELISA–> IFNy test = brucellose
Brucella suis
reportable and zoonotic
reservoir is wild boar biovar 1-2= reservoir in rabbits, biovar 3= pigs
Brucella suis Cs in swine
abortion, orchitis, infertility
arthiritis, bursitis, osteomyelitis
Brucella suis CS in human
acute- rise and fall in temp, sweating, chills, malaise, joint pain
osteomyelitis
localization, necrosis, pus- chronic
Brucella suis pathogenesis
skin/mucosa–> regional lymoh nodes –> bacteremia –> localization and abcess
more pathogenic in humans and B.abortus
Dx of brucellosis
contact with bovines (B.abortus) yersinia enterocolitica O9 brucella suis serology lots of cross reaction
B. melitensis
sheep and goat
in subtropical and mediterranean
principle cause for brucellosis in humans no vx
no O Ag, obligate parasitic, obligate pathogen
B.canis
long bacteremia
no fever
late abortion, lymphandenitis, late abortion, vaginal discharge, epididmitis, scrotal dermatitis, atrophia testicularis, prostatitis, STD
