enzymes that manipulate DNA 4A

Question 1

endonuclease

Answer 1

enzyme that breaks the phosphodiester bond between 2 nucleotides

Question 2

recognition site

Answer 2

target of DNA in which restriction endonuclease act

Question 3

restriction endonuclease

Answer 3

any enzyme that acts like molecular scissors to cut nucleic acid strands at a specific recognition site

Question 4

sticky ends

Answer 4

result of a staggered cut through double helix strand of DNA by an endonuclease resulting in over hanging nucleotides

Question 5

blunt end

Answer 5

result of a straight cut across double stranded DNA by endonuclease resulting in no overhanging nucleotides.

Question 6

ligase

Answer 6

liaise enzyme catalyses the formation of phosphodiester bonds in between nucleotides

Question 7

DNA polymerase

Answer 7

replicates or amplifies DNA, adds nucleotides to DNA.

Question 8

primer

Answer 8

short strand of nucleic acids that acts as a starting point for polymerase enzymes to attach.