CRISPR-Cas9 4B

Question 1

CRISPR-CAS9

Answer 1

complex formed between sgRNA and cas9 which can cut specific sequence of DNA

Question 2

bacteriophage

Answer 2

virius that infect prokaryotic cells

Question 3

CRISPR

Answer 3

custard regulatory interspaced short palindromic repeats

Question 4

gene knock out

Answer 4

when a gene is deactivated removed or silenced entirely from the DNA host

Question 5

gene knock in

Answer 5

inserting gene for another at the same gene loci in DNA host such as nucleotide

Question 6

cell repair mechanism

Answer 6

cells doesn’t want damaged DNA so it automatically attempts to fix damaged DNA, this is done is a rush so its prone to mistakes

Question 7

sgRNA

Answer 7

guides cas9 where to make its specific cut

Question 8

cas9

Answer 8

a restriction enzyme which act like molecular sizers to cut

Question 9

protospacer

Answer 9

fragment of DNA which is cut from the invading bacteriophage

Question 10

PAM sequence

Answer 10

the PAM sequence is like a little flag, just upstream the DNA to be cut, indicating where cas9 should make its cut.