ENZYMES: effect of temp, conc and pH on reaction rate

Question 1

Best functioning temperature/pH?

Answer 1

optimum temperature

Question 2

do optimum temperatures vary between organisms?

Answer 2

yes

Question 3

what happens when you increase the temperature past the optimum temp?

Answer 3

enzyme will become denatured -increased kinetic energy causes bonds in enzyme to break, changing shape of active site - not complementary or functional. RAPID DECREASE IN ROR

Question 4

what happens when you increase the temperature up to the optimum temp?

Answer 4

enzyme is at first INACTIVATED: enzyme and substrate collide with less energy, meaning they are less likely to overcome the activation energy barrier – resulting in a lower rate of reaction.
GRADUALLY INCREASES ROR

Question 5

do optimum ph’s of enzymes vary according to the environment that it usually operates in?

Answer 5

yes

Question 6

what happens when the pH is too high or low?

Answer 6

the enzyme can be DENATURED. The enzyme’s bonds can be altered, and thus 3-ary structure and shape of the active site can be changed. The enzyme is no longer complementary to the substrate and is no longer functional.

Question 7

effect of substrate concentration on reaction rate?

Answer 7

• Increases the rate of reaction until all active sites are occupied – the rate of reaction becomes constant. No. active sites becomes a limiting factor.

Question 8

effect of enzyme concentration of reaction rate?

Answer 8

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