enzymes and metabolism Flashcards
metabolism
all the chemical reactions that take place in cells
what are the 2 types of metabolism?
catabolism and anabolism
catabolism
large molecules are broken down into smaller ones
eg of catabolism
digestion
anabolism
small molecules are built up into bigger ones
eg anabolism
protein synthesis
requires energy
anabolism
releases energy
catabolism
enzymes
catalyst proteins that allow chemical reactions to occur at a fast enough rate at body temperature for the body to function
- enzymes :
are specific and only combine with 1 particular substrate at the active site
- enzymes:
enzymes and substrates are complimentary. once combined they form a enzymes substrate complex
activation energy
reacting particles need to collide with enough energy to break the bonds.
also must collide with the right atoms
how does the concentration of enzymes affect enzyme function
- the higher the concentration the faster the rate of reaction
- there are more enzymes to influence reactants
- the body controls the rate of reaction by regulation the no. of enzymes
how does the concentration of substrates affect enzyme function
-the higher the substrate concentration the faster the rate of reaction
- more substrates are contacting enyme molecules
- increasing beyond a certain point has no effect because active sites in enzymes are fully occupied
how does the removal of products affect enzyme function
-products of reaction must continually be removed or it will slow as it becomes more difficult to come into contact with the enzymes
how does the temperature affect enzyme function
-the higher the temperature the higher the rate of reaction
-only to 45-50 degrees or they loose their shape and become denatured
-optimum temperature is 30-40 degrees
denatured
when enzymes get too hot they lose their shape and the ability to bind to a substrate so they become inactive
how does the pH affect enzyme function
- enzymes are very sensative to pH
- each enzymes has an optimum pH level they work best at
what are cofactors?
ions that need to be present for enzymes to function
how do cofactors affect enzyme function
- they change the shape of the active site so enzymes can combine with substrates
- without cofactors enzymes cannot function
eg of cofactors
vitamins
how do the enzymes inhibitors affect enzyme function
- they are substances that slow and stop enzyme activity
- used to control the reactions
eg of enzyme inhibitors
drugs such as penicillin
- prevents enzyme activity in bacteria that is involved in the constuction of the cell wall