Enzymes and Energy

Question 1

Free-energy
positive delta G=_____
negative delta G=_____

Answer 1

positive delta G=non-spontaneous
negative delta G= Spontaneous

Question 2

Metabolism

Answer 2

An organisms chemical reactions
catabolic=exergonic; release of energy
anabolic=endergonic; energy-consuming

Question 3

Catabolism

Answer 3

molecular breakdown
-Hydrolysis
-energy released
-negative delta G (spontaneous)

Break-down of nutrients from food=digestion

Question 4

Anabolism

Answer 4

molecular synthesis
-Dehydration/Condensation Rxns
-energy consumed
-positive delta G

Nutrients absorbed through the intestine and taken up by cells to build new protein structures.

Question 5

Coupled Reactions

Answer 5

Rxns in which you use exergonic energy (ATP hydrolysis) to drive endergonic processes

Question 6

How do enzymes speed up reactions?

Answer 6

By lowering activation energy
-biological catalysts

Question 7

Clicker:
What do you think would happen if you put chymotrypsin in extremely low pH (2)?

A. It denatures and won’t catalyze peptide hydrolysis.
B. It will catalyze peptide hydrolysis at low pH, just as it would at neutral pH.
C. It denatures and will catalyze peptide hydrolysis.
D. It will catalyze peptide hydrolysis at low pH better than at higher pH.
E. The primary amino acid sequence changes.

Answer 7

a.) It denatures and wont catalyze peptide hydrolysis
normal function of Chymotrypsin=hydrolysis of peptide sequence at c-term of specific aa’s ; if its denatured, it can no longer serve its normal function