enzyme regulation

Question 1

two general ways to regulate enzymes

Answer 1

activity
availability

Question 2

activity control

Answer 2

how active the set of enzymes are…
controlled by:
- reversible covalent modification
- reversible allosteric modification
- binding to regulatory protein
- inhibition by competitive, uncompetitive or mixed inhibitors
- substrate level control
- feedback inhibition

Question 3

reversible covalent modification

Answer 3

type of activity control…
often the addition or removal of Phosphate
(kinase and phosphatase)…
typically serine, tyrosine and threonine but focus more on serine

Question 4

allosteric modification

Answer 4

can either be inhibition or activation. STUDY THE IMAGE

Allosteric binding allows substrate to bind worse or better

Question 5

binding to regulatory protein

Question 6

substrate level control

Answer 6

direct action by substrate OR product (can be a substrate or the final product) FROM THAT PATHWAY on the enzyme

Question 7

feedback control

Answer 7

something further downstream or in another rxn that is connected…

Question 8

availability control

Answer 8

how much enzyme do i have?
- includes whats inhibited, active, or hidden/sequestered.

Ratio of enzyme synthesis to enzyme degredation…

also need to think about sequestration which is hiding an enzyme is another compartment.