Enzyme Expreriments Flashcards
pH exp. Name an enzyme used
Catalase
pH product
H²0 + O²
pH independent variable (How was it varied)
The pH
- Varied by 4cm³
pH constants. How were they kept constant?
Hydrogen Peroxide, Celery
- Measured in grad cyl
- Measured in syringe
pH Dependent variable
Volume of foam
pH How was the rate of reaction recorded?
Measure volume of foam
pH Control?
No celery in one (therefore no enzymes)
pH Optimum pH
9.22
What is an active site
Where the enzymes attached to the substrates
Temp Enzyme Used
Catalase as well
Temp product
Foam
Temp What was varied. How was it varied
Temperature. 10° - 60° C
Temp Constants. How kept constant
pH and Celery
- Grad Cyl
Temp Control
One without celery
Temp. How does heat affect the activity of the enzyme
The highest and lowest temperatures have the smallest change
Temp. If the temperature rises over 60°c, what happens to the active site?
The enzymes become denatured and lose shape and function
Temp. How was the rate of activity measured?
Volume of foam
Temp. What was the optimum temperature?
30°C
Heat Dena. Enzyme used
Catalase
Heat Dena. Substrate and product
H2O2 and foam in cm³ (O2 and H20)
Heat Dena. Constants. How were they kept constant?
Hydrogen peroxide and pH 9
- Measured in syringe and grad cyl
Induced fit model of active site?
The enzyme changes shape to fit the substrate
Heat Dena. Result and conclusion
Denatured Enzyme didn’t react.
Denatured enzymes will not work anymore
What is the difference between denatured enzymes and inactive enzymes?
Dena - lost shape and function
Ina - not functioning temporarily
