Enzyme Expreriments

Question 1

pH exp. Name an enzyme used

Answer 1

Catalase

Question 2

pH product

Answer 2

H²0 + O²

Question 3

pH independent variable (How was it varied)

Answer 3

The pH
- Varied by 4cm³

Question 4

pH constants. How were they kept constant?

Answer 4

Hydrogen Peroxide, Celery
- Measured in grad cyl
- Measured in syringe

Question 5

pH Dependent variable

Answer 5

Volume of foam

Question 6

pH How was the rate of reaction recorded?

Answer 6

Measure volume of foam

Question 7

pH Control?

Answer 7

No celery in one (therefore no enzymes)

Question 8

pH Optimum pH

Answer 8

9.22

Question 9

What is an active site

Answer 9

Where the enzymes attached to the substrates

Question 10

Temp Enzyme Used

Answer 10

Catalase as well

Question 11

Temp product

Answer 11

Foam

Question 12

Temp What was varied. How was it varied

Answer 12

Temperature. 10° - 60° C

Question 13

Temp Constants. How kept constant

Answer 13

pH and Celery
- Grad Cyl

Question 14

Temp Control

Answer 14

One without celery

Question 15

Temp. How does heat affect the activity of the enzyme

Answer 15

The highest and lowest temperatures have the smallest change

Question 16

Temp. If the temperature rises over 60°c, what happens to the active site?

Answer 16

The enzymes become denatured and lose shape and function

Question 17

Temp. How was the rate of activity measured?

Answer 17

Volume of foam

Question 18

Temp. What was the optimum temperature?

Answer 18

30°C

Question 19

Heat Dena. Enzyme used

Answer 19

Catalase

Question 20

Heat Dena. Substrate and product

Answer 20

H2O2 and foam in cm³ (O2 and H20)

Question 21

Heat Dena. Constants. How were they kept constant?

Answer 21

Hydrogen peroxide and pH 9
- Measured in syringe and grad cyl

Question 22

Induced fit model of active site?

Answer 22

The enzyme changes shape to fit the substrate

Question 23

Heat Dena. Result and conclusion

Answer 23

Denatured Enzyme didn’t react.
Denatured enzymes will not work anymore

Question 24

What is the difference between denatured enzymes and inactive enzymes?

Answer 24

Dena - lost shape and function
Ina - not functioning temporarily

Question 25

Heat Dena. Control

Answer 25

The fresh celery

Question 26

Can an enzyme be denatured in another way? Explain

Answer 26

Yes.
Unsuitable pH.

Question 27

Enzyme imo. What is an immobilised enzyme?

Answer 27

Enzyme that is stuck to an inert material.

Question 28

Enzyme imo. The enzyme that was immobilised and outline reaction it catalysed

Answer 28

• Sucrase
• Sucrose → Glucose

Question 29

Enzyme Imo. What is a bioreactor?

Answer 29

Vessel in which a biological reaction occurs

Question 30

Enzyme imo. What is the gel made of?

Answer 30

Sodium aliginate

Question 31

Enzyme imo. What are the beads dropped into? Why are they dropped in?

Answer 31

Calcium chloride.
- Helps form the beads

Question 32

Enzyme imo. What is bio processing?

Answer 32

Making products with living things