Enzyme Expeirmebt 1

Question 1

Expeirmebt for enzyme

Answer 1

Dilute stock trypsin solution with dialled water to produce solution ranging in concentration rom 0.2% till 0.8%
Add a ct9nrol by adding 2cm3 of distilled water and 2cm3 of trypsin solution add to colorimeter to make sure colorimeter solution is 0
Add to another corvette 2cm3 of milk suspension and 2cm3 of stock trypsin solution
Mix Add to colorimeter
Measure absorbance every 15 second for 5 minutes
Repeat at different trypsin concentrations

Question 2

Conclusion 1 about milk

Answer 2

Contains causing which when broken down makes milk countless, trypsin is a protease enzyme which beaks it down

Question 3

Conclusion 2 about rate of rwcation

Answer 3

As concentration of trypsin increases, number of enzyme substrate complexes increases because enzymes and substrate are more.likely to collide with each other therfore increasing ror

Question 4

Conclusion 3 leveling off

Answer 4

The rate levels off as all enzymes active sites is saturated.
Increasing enzyme conctration won’t help as enzyme concatrtion is limiting factor