ELISA

Question 1

What are the steps of ELISA?

Answer 1

1. Coating: immobilization of antigens to the microplate wells
2. Plate Blocking: adding an irrelevant protein or molecule to bind to unsaturated surface-binding sites of the wells
3. Probing: Incubation with antigen-specific antibodies that will affinity-bind to the antigen
4. Signal Measurement: Detect the signal generated by a direct or secondary tag on the specific antibody

Question 2

What is used to read ELISA?

Answer 2

A spectrophotometer