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MICRO2209 > ELISA > Flashcards

ELISA Flashcards

1
Q

What does ELISA stand for?

A

Enzyme linked immunosorbent assay

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2
Q

What is ELISA used for?

A

enables the analysis of antibodies, antigens, proteins and glycoproteins

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3
Q

How are ELISA’s performed on?

A

polystyrene microplate plates

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4
Q

How do you setup an ELISA?

A
  1. Microtiter wells coated with antigen
  2. Specific antibody present, forming antigen-antibody complex
  3. Reactions are detected using a second enzyme-linked antibody
  4. Addition of substrate causes positive reactions
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MICRO2209 (16 decks)

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