blood smear Flashcards
1
Q
What is a commonly used stain?
A
May-Grunwald-Giemsa or Wright’s quick blood stains
2
Q
Why is a blood smear used before differential staining?
A
Ensure cells are fixed.
Chemical groups present and DNA are not altered
3
Q
How do we ensure no chemical alteration and adhesion of cells to a glass slide?
A
Using alcohol instead of a reagent
4
Q
What are the steps to prepare a blood smear?
A
1`. Small drop of blood placed on one end of an alcohol-cleaned microscopic slide
- Another slide is inclined at 45 degrees and drop contacts and spread along the edge
- Slide is pushed smoothly along the other slide = spread out in an even manner
