DNA6 Flashcards
The major and minor grooves of DNA have specific …… potential and …..
H-bonds
shape
Define specificity.
delta delta G; the difference in affinity between a perfect match and a mismatch
What can nature do to add more specificity to a biological unit?
Use precisely spaced units that are connected by turn helixs
define informatics
the study of sequences
What type of electrostatic charge do histones have?
positive
Are histones acidic or basic?
basic - positively charged.
What are intercalators?
planar, pi stacked molecules like dyes that recognize subsequences and stretch DNA vertically
What is the purpose of intercalation?
To stop translation
How can you separate DNA duplexes?
heating stress
mechanical stress
What is the name of the field of study that focuses on the linking number (write and twist) of DNA?
Topology
What enzymes can untwist nucleic acid duplexes?
helicase, gyrase, topoisomerases
What synthesizes the small RNA primer on the template strand?
primase
What does methylation signal?
parent strand and which strand is to be repaired if damaged or mutated
What are the two names of the molecule that can remove incorrectly synthesized base pairs or other mistakes?
nucleases
polymerase complex
Polymerization proceeds by ….. ……
nucleophillic attack
